Scorpion venom, with its constituents, has the potential to be a drug candidate. Scorpion venom is a mixture of biologically active compounds, including enzymes, toxic peptides, free amino acids, and other metabolites. In this study, the peptide maps and metabolic analysis of the venoms will be elucidated by transcriptomic analysis of mRNA profiles. Tissues were obtained from the venom glands of the scorpion species Androctonus crassicauda, Hottentotta saulcyi, and Leiurus abdullahbayrami. The mRNA was isolated from venom tissues obtained from the venom glands of the different scorpion species. As a result, 8 different proteins were obtained from the species A. carsicauda and H. saulcyi, a nd 7 d ifferent p roteins from the species L. abdullahbayrami. According to the LC-MS/MS results, the amino acids taurine, alpha-aminobutyric acid, phosphoethanolamine, lysine, leucine, and arginine were detected in the scorpion venom of A. crassicauda. H. saulcyi; the amino acids taurine, lysine, phosphoethanolamine, arginine, alpha-aminopimelic acid, and leucine were detected in scorpion venom. L. abdullahbayrami; alpha- aminobutyric acid, taurine, lysine, phenylalanine, arginosuccinic acid, leucine, histamine, arginine, proline amino acids were detected in the scorpion venom. It is important to emphasize that this study not only adds to our existing knowledge of scorpion venoms, but may also serve as a basis for future research to define the composition of scorpion venoms and facilitate the identification of new putative toxin families.