Our paper presents a range of research carried out for environment protection and management using GIS applications. It documents 20 years of scientific achievements through Polish-Croatian cooperation in the GIS field. The resultant body of work includes, inter alia, scientific publications that are the result of 20 international conferences organized in the framework of said cooperation efforts. The study included articles published in 1994 to 2012 and those drafted for publication in 2013. An analysis of select articles was done in accordance with the accepted range of topics in order to categorize and define the research extent. Among the distinguished 19 subject categories, most of the articles were concerned with: environmental management, nature conservation, landscape management, biodiversity, sustainable development, meteorology, and climatology.