ORIGINAL RESEARCH
A Comparative Assessment of Three Pollen Substitutes for Honey Bee (Apis Mellifera L.) During Winter and Spring
 
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1
Department of Arid Land Agriculture (Plant Protection Science Program); College of Agri. & Food Sciences. King Faisal University; P.O Box 55073, Hofuf 31982, Saudi Arabia
 
2
Honey bee Research Section, Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
 
3
Department of Economic Entomology, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
 
 
Submission date: 2024-03-12
 
 
Final revision date: 2024-04-18
 
 
Acceptance date: 2024-05-02
 
 
Online publication date: 2024-11-18
 
 
Publication date: 2025-01-28
 
 
Corresponding author
Nabil M. Elwakeil
Department of Arid Land Agriculture (Plant Protection Science Program); College of Agri. & Food Sciences. King Faisal University; P.O Box 55073, Hofuf 31982, Saudi Arabia
 
 
El-Kazafy A. Taha   

Department of Economic Entomology, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh 33516, Egypt, Egypt
 
 
Pol. J. Environ. Stud. 2025;34(3):3121-3128
 
KEYWORDS
TOPICS
ABSTRACT
Pollen satisfies the nutritional requirements for developing honey bees, and without pollen, there is no brood development. Beekeepers feed colonies pollen substitutes or pollen supplements to stimulate brood rearing in the winter and early spring, or when pollen from blooming plants is scarce. The performance of honey bee colonies has been enhanced by supplementation with pollen substitutes. The current study aimed to evaluate the efficiency of three pollen substitutes in maintaining the colony strength during winter and improvement of colony performance during the winter and spring seasons. The three diets consisted of 30 g peeled white lupine flour + 20 g brewer’s yeast + 20 g casein milk powder + 20 g honey + 10 g sugar powder (diet 1), 30 g fenugreek flour + 20 g brewer’s yeast + 20 g casein milk powder + 20 g honey + 10 g sugar powder (diet 2), and 30 g defatted soybean flour + 20 g brewer’s yeast + 20 g casein milk powder + 20 g honey + 10 g sugar powder (diet 3), and were provided to the experimental colonies in a paste form and compared with the unfed colonies. Diet 1 was the most accepted and consumed diet by honey bee workers during the winter and spring seasons. Colonies fed on diet 1 stored pollen, reared worker brood, and had adult population sizes larger than colonies fed on diet 2, diet 3, and unfed colonies. Colonies fed on diet 1, diet 2, and diet 3 produced more honey than the unfed colonies by 125.00%, 95.00%, and 70.00%, respectively. Diet 1 could be recommended to feed honey bee colonies during winter and spring to sustain the strength of the colonies and improve their productivity.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
REFERENCES (43)
1.
SEELEY T.D. Honey bee ecology. Princeton University Press. Princeton, New Jersey pp. 202, 1985.
 
2.
TAHA E.-K.A., AL-KAHTANI S.N., TAHA R. Protein content and amino acids composition of bee-pollens from major floral sources in Al-Ahsa, eastern Saudi Arabia. Saudi Journal of Biological Sciences, 26, 232, 2019. https://doi.org/10.1016/j.sjbs... PMid:31485159 PMCid:PMC6717098.
 
3.
TAHA E.-K.A., AL-KAHTANI S.N. Macro- and trace elements content in honey bee pollen loads in relation to the harvest season. Saudi Journal of Biological Sciences, 27, 1797, 2020.
 
4.
AL-KAHTANI S.N., TAHA E.-K.A., FARAG S.A., TAHA R., ABDOU E., MAHFOUZ H. Harvest season significantly influences the fatty acid composition of bee pollen. Biology, 10, 482, 2021.
 
5.
AL NAGGAR Y., TAHA I.M., TAHA E.-K.A., ZAGHLOOL A., NASR A., NAGIB A., ELHAMAMSY S.M., ABOLABAN G., FAHMY A., HEGAZY E., METWALY KH.H., ZAHRA A.A. Gamma irradiation and ozone application as preservation methods for longer-term storage of bee pollen. Environmental Science and Pollution Research, 2024 https//doi.org/10.1007/s11356-024-32801-4.
 
6.
TAHA I.M., ISMAIL K.A., SAMI R., ALJUMAYI H., ALSHEHRY G., ALGARNI E., ALJUMAYI A., ALSHAHRANI M.Y., ASSERI T.A.Y., ALSHARARI Z.D., BAAKDAH F., TAHA E.-K.A. Bee pollen, an antioxidant and antimicrobial agent for beef sausage. Journal of Biobased Materials and Bioenergy, 18, 31, 2024.
 
7.
ISRAR M., MALIK S., ALI J., KHAN M., KHAN K., TARIQ K., ALAM S. Effect of artificial pollen substitutes diets on diets consumption, brood area, and colony strength of Apis mellifera L. colonies during dearth period. Journal of Applied Entomologist, 2, 9, 2022.
 
8.
SAFFARI A., KEVAN P.J., ATKINSON J. Palatability and consumption of patty, formulated pollen and pollen substitutes and their effects on honey bee colony performances. Journal of Apicultural Science, 54, 63, 2010.
 
9.
SHAWER M.B, TAHA E.-K.A., MOUSA K.M., KHAN K.H., IBRAHIM S., HASSAN S., ELNABAWY E.M. Seasonal variations of colony activities linked to morphometric and glands characterizations of hybrid Carniolan honey bee (Apis mellifera carnica Pollmann) workers. Journal of King Saud University- Science, 33, 101543, 2021.
 
10.
MATILLA H.R., OTIS G.W. Influence of pollen diet in spring on the development of the honey bee (Hymenoptera, Apidae) colonies. Journal of Economic Entomology, 99, 604, 2006. https://doi.org/10.1093/jee/99... PMid:16813288.
 
11.
TAHA E.-K.A. The impact of feeding certain pollen substitutes on maintaining the strength and productivity of honey bee colonies (Apis mellifera L.). Bulletin of Entomological Society of Egypt Economic Series, 41, 63, 2015.
 
12.
GHRAMH H.A., KHAN K.A. Honey bees prefer pollen substitutes rich in protein content located at short distance from the Apiary. Animals, 13, 885, 2023. https://doi.org/10.3390/ani130... PMid:36899742 PMCid:PMC10000118.
 
13.
ROUSSEAU A., GIOVENAZZO P. Optimizing drone fertility with spring nutritional supplements to honey bee (Hymenoptera, Apidae) colonies. Journal of Economic Entomology, 109, 1009, 2016. https://doi.org/10.1093/jee/to... PMid:27018435.
 
14.
SCHMICKL T., CRAILSHEIM K. (How honey bees (Apis mellifera L.) change their brood care behavior in response to non-foraging conditions and poor pollen conditions. Behavioral Ecology and Sociobiology, 51, 415, 2002.
 
15.
HUANG Z. Pollen nutrition affects honey bee stress resistance. Terrestrial Arthropod Reviews, 5, 175, 2012. https://doi.org/10.1163/187498....
 
16.
AQUEEL M.A., ABBAS Z., SOHAIL M., ABUBAKAR M., SHURJEEL H.K., RAZA A.B.M., AFZAL M., 3128 Nabil M. Elwakeil, et al. ULLAH S. Effect of varying diets on growth, development and survival of queen bee (Apis mellifera L.) in captivity. International Journal of Agricultural and Biosystems Engineering, 10, 888, 2017. https://doi.org/10.1002/term.2....
 
17.
PUŠKADIJA Z., SPILJAK L., KOVAČIĆ M. Late winter feeding stimulates rapid spring development of carniolan honey bee colonies (Apis mellifera carnica). Poljoprivreda, 53, 54, 2017.
 
18.
WIJAYATI N., HARDJONA D.S., RAHMAVATI M., KURNIAWATI, A. Formulation of winged bean seeds as pollen substitute for outgrowth of honey bees (Apis mellifera L.). Journal of Physics: Conference Series, 1321, 022040, 2019.
 
19.
ISLAM N., MAHMOOD R., SARWAR G., AHMED S. Development of pollen substitute diets for Apis mellifera ligustica colonies and their impact on brood development and honey production. Pakistan Journal of Agricultural Research, 33, 381, 2020. https://doi.org/10.1108/ARJ-01....
 
20.
KHAN KH.A., GHRAMH H.A. Nutritional efficacy of different diets supplemented with microalga Arthrospira platensis (spirulina) in honey bees (Apis mellifera). Journal of King Saud University- Science, 34, 101819, 2022. https://doi.org/10.1016/j.jksu....
 
21.
TAHA E.-K.A., TAHA R., AL-KAHTANI S.N. Nectar and pollen resources for honey bees in Kafrelsheikh, Northern Egypt. Saudi Journal of Biological Sciences, 26, 890, 2019.
 
22.
TAHA E.-K.A., AL-KAHTANI S.N. Comparison of the activity and productivity of Carniolan (Apis mellifera carnica Pollmann) and Yemeni (Apis mellifera jemenitica Ruttner) subspecies under environmental conditions of the Al-Ahsa oasis of eastern Saudi Arabia. Saudi Journal of Biological Sciences, 26, 681, 2019. https://doi.org/10.1016/j.sjbs... PMid:31048992 PMCid:PMC6486514.
 
23.
TAHA E.-K.A., AL-KAHTANI S.N. Relationship between population size and productivity of honey bee colonies. Journal of Entomology, 10, 163, 2013. https://doi.org/10.3923/je.201....
 
24.
KAVITHA T., KATHIRVELU C., MUTHUKRISHNAN N., GOPIANAND L. Influence of Weather factors on colony growth of Indian honey bee, Apis cerana indica Fab. in Annamalainagar, Tamil Nadu, India. Indian Journal of Natural Science, 12, 36370, 2021.
 
25.
TAHA E.-K.A., AL-KAHTANI S.N. The relationship between comb age and performance of honey bee colonies. Saudi Journal of Biological Sciences, 27, 30, 2020. https://doi.org/10.1016/j.sjbs... PMid:31889813 PMCid:PMC6933216.
 
26.
AL-KAHTANI S.N., TAHA E.-K.A. Effect of comb age on cell measurements and worker body size. PloS One, 16, e 0260865, 2021.
 
27.
TAHA E.-K.A., RAKHA O.M., ELNABAWY S.M., HASSAN M.MM., SHAWER D.M.B. Comb age significantly influences the productivity of the honey bee (Apis mellifera) colony. Journal of King Saud University - Science, 33, 101436, 2021.
 
28.
TAHA E.-K.A., SHAWER M.B., TAHA R., ELASHMAWY A., GABER S., MOUSA K. Comb age significantly influences the emergency queen rearing, morphometric and reproductive characteristics of the queens. Journal of Apicultural Research, 2024. https://doi.org/10.1080/0021 8839.2024.2336376.
 
29.
AOAC. Official methods of analysis (17th ed.) Washington, DC., USA, Association of Official Analytical Chemists, 2000.
 
30.
TAHA E.-K.A. Importance of banana Musa sp. (Musaceae) for honey bee Apis mellifera L. (Hymenoptera, Apidae) in Egypt. Bulletin of Entomological Society of Egypt II, 125, 2007.
 
31.
SAS INSTITUTE SAS/STAT User's Guide release 9.1. SAS Institute Inc, Cary, NC 27513, 2003.
 
32.
AL-KAHTANI S.N., TAHA E.-K.A., KHAN KH.A., ANSARI M.J., FARAG S.A., SHAWER D.M.B., ELNABAWY S.M. Effect of harvest season on the nutritional value of bee pollen protein. PloS One, 15, e0241393, 2020. https://doi.org/10.1371/journa... PMid:33370277 PMCid:PMC7769433.
 
33.
TAHA E.-K.A. Chemical composition and amounts of mineral elements in honey bee-collected pollen in relation to botanic origin. Journal of Apicultural Science, 59, 75, 2015. https://doi.org/10.1016/j.repl....
 
34.
SPULBER R., DOĞAROĞLU M., BĂBEANU N., POPA O. Physicochemical characteristics of fresh bee pollen from different botanical origins. Romanian Biotechnological Letters, 23, 13357, 2018. https://doi.org/10.5465/AMBPP.....
 
35.
AL-KAHTANI S.N., TAHA E.-K.A. Seasonal variations in nutritional composition of honey bee pollen loads. Journal of the Kansas Entomological Society, 93, 105, 2020.
 
36.
AYLANC V., FALCAO S.I., VILAS-BOAS M. Bee pollen and bee bread nutritional potential, Chemical composition and macronutrient digestibility under in vitro gastrointestinal system. Food Chemistry, 413, 135597, 2023. https://doi.org/10.1016/j.food... PMid:36791664.
 
37.
TAHA E.-K.A. Honey bee and modern beekeeping. Translation, Authoring and Publishing Center King Faisal University, Al-Ahsa, Saudi Arabia. 2013 (In Arabic).
 
38.
POKHREL S., THAPA R.B., NEUPANE F.P., HRESTHA S.M. Absconding behavior and management of Apis cerana F. honey bee in Chitwan, Nepal. Journal of the Institute of Agriculture and Animal Science, 27, 77, 2006. https://doi.org/10.3126/jiaas.....
 
39.
TAHA E.-K.A., AL-ABDULSALAM M., AL-KAHTANI S.N. Insect pollinators and foraging behavior of honey bees on alfalfa (Medicago sativa L.) in Saudi Arabia. Journal of the Kansas Entomological Society, 89, 92, 2016. https://doi.org/10.2317/150402....
 
40.
ULLAH A., SHAZAD M.F., IQBAL J., BALOCH M.S. Nutritional effects of supplementary diets on brood development, biological activities and honey production of Apis mellifera L. Saudi Journal of Biological Sciences, 28, 6861, 2021.
 
41.
TOPAL E., M˘ARG˘AOAN R., BAY V., TAKMA Ç., YÜCEL B., OSKAY D., DÜZ G., ACAR S., KÖSO˘GLU M. The effect of supplementary feeding with different pollens in autumn on colony development under natural environment and in vitro lifespan of honey bees. Insects, 13, 588, 2022.
 
42.
HELAL R.M., EL-DAKHAKHNI T.N., SHAWER M.B., TAHA E.-K.A. Effect of moving the apiaries on activity of honey bee colonies. 2- Flight activity, gathering of nectar and sugar concentration contents and honey. Journal of Agricultural Research Tanta University, 29, 268, 2003.
 
43.
SHAWER M.B., EL-DAKHAKHNI N.M., HELAL R.M., TAHA E.-K.A. Effect of moving the apiaries on activity of honey bee colonies. 1- Gathering and storing pollen, brood rearing and wax secretion. Journal of Agricultural Research Tanta University, 29, 250, 2003.
 
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