This study focuses on the evaluation of antioxidant activity, phenolic and flavonoids contents of selected flaxseed cultivars, native to Pakistan. Antioxidant activities were determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric ion reducing ability and inhibition of linoleic acid peroxidation (LAP). Total phenolic content (TPC) was determined using Folin- Ciocalteu reagent, whereas colorimetric method was used to assess total flavonoids (TF). The TPC, TF, DPPH˚ scavenging activity and LAP were 2560 to 3286 mg gallic acid equivalent (GAE)/100 g, 232.53 to 346.67 mg catechin equivalent (CE)/100g and 63.08 to 86.58%, 65.59 to 84.25%, respectively. Results of the present study revealed that flaxseed cultivars possess varying degree of antioxidant capacity and therefore may be considered as a potent source of natural antioxidants.