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ORIGINAL RESEARCH
Assessing the Biocontrol Potential of Some
Isolated Bacteriophages Against Salmonella spp.
in Food Preservation: A Preliminary Study
1
Department of Chemistry, Faculty of Science, Cairo University, Cairo, Egypt
2
Animal Production Department, Food and Agriculture Sciences College, King Saud University, Riyadh, Saudi Arabia
3
Toxicology and Mycotoxin Research Unit, U.S. National Poultry Research Center, Agricultural Research Service, U.S.
Department of Agriculture, Athens, GA, United States
4
The Regional Center for Mycology and Biotechnology, Al-Azhar University, Nasr City, Cairo, Egypt
Submission date: 2024-01-18
Final revision date: 2024-03-10
Acceptance date: 2024-05-19
Online publication date: 2024-09-04
Publication date: 2025-04-04
Corresponding author
Mohammed Yosri
The Regional Center for Mycology and Biotechnology, Al-Azhar University, Nasr City, Cairo, Egypt
The Regional Center for Mycology and Biotechnology, Al-Azhar University, Nasr City, Cairo, Egypt
Pol. J. Environ. Stud. 2025;34(4):3763-3777
KEYWORDS
TOPICS
ABSTRACT
Food- and water-borne illnesses caused by Salmonella spp. are one of the pandemic loads
in developing nations and are seen as a sign of poor food and water cleanliness. In the early 20th
century, bacteriophages were often utilized to treat various bacterial illnesses, but their significance
declined after the development of pharmaceutical antibiotics. The resurgence of several microorganisms
with antibiotic resistance has sparked new interest in this field of study. This research was done
to evaluate the effectiveness of domestically acquired bacteriophages utilized as effective bio-control
agents and Salmonella spp. detection methods. Using the enhanced culture approach, 28 phages
were recovered from environmental materials. Host range analysis, restriction analysis, pulsed-field
gel electrophoresis (PFGE), and transmission electron microscopy were used to characterize some
of the isolated phages. Isolated phages may have the potential to be a safe and efficient substitute for
antibiotics in the fight against Salmonella infection in the food business since they can be used to
biocontrol the bacterium Salmonella for food preservation without changing the natural flora of the
gastrointestinal tract. The CUMR17 phage from the family Siphoviridae was chosen due to its size
for the biocontrol of Salmonella spp. in chicken meat and milk at 4ºC and 25ºC, with a constant
and powerful biocontrol impact even prior to 24 hours at 4ºC. The findings may be used to create
a biocontrol agent that avoids Salmonella infection in the food sector, making food safer in impoverished
nations.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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