ORIGINAL RESEARCH
Counteracting Heat, Salinity, and Osmotic Stresses by Reconciling Seed Size and Sowing Depth for Bolstering Germination and Seedling Growth of Cluster Bean (Cyamposis tetragonoloba L. Taub.)
 
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1
Department of Agronomy, Faculty of Agriculture, University of Agriculture Faisalabad, Faisalabad 38040, Pakistan
 
2
Department of Field Crops, Faculty of Agriculture, Siirt University, Turkey
 
3
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
 
4
Plant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia
 
5
Department of Chemical Engineering, Louisiana Tech University, Ruston LA 71270, United States
 
 
Submission date: 2024-06-02
 
 
Final revision date: 2024-06-27
 
 
Acceptance date: 2024-10-27
 
 
Online publication date: 2024-11-21
 
 
Publication date: 2025-11-14
 
 
Corresponding author
Muhammad Aamir Iqbal   

Department of Chemical Engineering, Louisiana Tech University, Ruston LA 71270, United States
 
 
Pol. J. Environ. Stud. 2025;34(6):8029-8041
 
KEYWORDS
TOPICS
ABSTRACT
Under abiotic stresses (AS, heat, salinity, and osmotic stresses), seed germination and seedling growth of legumes like cluster beans are critical phases of the crop growth cycle that determine their productivity. Research gaps exist regarding the impacts of seed size, sowing depth, and soil pH on the germination, root, and shoot growth attributes of cluster beans when exposed to AS. Five different trials were executed to assess the comparative performance of cluster bean seed sizes (small, medium, and large) in response to different regimes of temperature (T, 10, 20, and 30°C), salinity (SS, 50, 100, 150, and 200 mM), osmotic stress (OS, 0.2, 0.4, 0.6, 0.8, and 1.0 Mpa), soil pH (5, 6, 7, 8, 9, and 10), and sowing depths (SD, 0, 2, 4, 6, and 8 cm). The response variables included germination (GP), germination index (GI), and time taken to 50% germination (TG), along with length, fresh, and dry weights of the shoot and root of the cluster bean. The results exhibited that large-size seeds sown at 4 cm depth remained unmatched by recording the maximum GP and GI up to 20-30°C temperature, 0-50 mM salinity level, 0-0.4 Mpa osmotic stress, and 6-7 pH. The same range of employed treatments also remained effective in a pronounced reduction of time taken to the TG. Moreover, cluster bean largesize seeds sown in 4 cm depth depicted the maximum root and shoot attributes, whereas smaller seed sizes sown in 0, 6, and 8 depths when exposed to 10°C temperature, 100-200 mM SS levels, soil pH (5, 9, and 10), and 0.6, 0.8, and 1.0 Mpa of OS recorded significantly reduced shoot and root traits. Thus, these findings reveal the feasibility of alleviating moderate levels of AS for cluster beans with large seed sizes and sowing depth reconciliation under semi-arid conditions.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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