Investigation of the Valorization of Methanolic Extract of Punica granatum L. Peel in Terms of Phytochemical, Trace Element, Antioxidant Activities and ADMET Profile of Active Compounds
More details
Hide details
Department of Biology, College of Science, University of Ha’il, P.O. Box 2440, Ha’il 2440, Saudi Arabia
Laboratory of Bioactive Substances, Center of Biotechnology of Borj Cedria, BP-901, 2050 Hammam-lif, Tunisia
University of Tunis-El Manar, Campus Universitaire Farhat Hached, BP-94 Rommana, 1068 Tunis, Tunisia
Center of Excellence in Desalination Technology, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia
Department of Computer Engineering, College of Computer Science and Engineering, University of Ha’il, KSA
Innov’COM, SupCom, Carthage University, Tunis, Tunisia
Section of Histology - Cytology, Medicine College of Tunis, Tunis El Manar University, Road Djebel Lakhdhar, 1007 La Rabta-Tunis, Tunisia
These authors had equal contribution to this work
Submission date: 2023-06-17
Final revision date: 2023-08-09
Acceptance date: 2023-11-08
Online publication date: 2024-02-09
Publication date: 2024-03-18
Corresponding author
Salem Elkahoui   

Department of Biology, College of Science, University of Ha’il, P.O. Box 2440, Ha’il 2440, Saudi Arabia
Pol. J. Environ. Stud. 2024;33(3):2587-2602
In this study, to valorize the pomegranate peels, the phytochemical composition of the methanolic extract of Punica granatum L. peel (PPME) was characterized by high-resolution liquid chromatographymass spectrometry (LC/HRMS), while the mineral composition of the extract was determined by ICP AES. In the methanolic pomegranate peel extract, thirty-five different compounds were identified by LC/HRMS, and it was determined that these compounds were mainly terpenoids, lipids and flavonoids. Total polyphenol, flavonoid, and tannins contents of the PPME extract were determined as 211.43 mg GAE/g polyphenol, 20.7 mg QE/g flavonoids and 11.5 mg TEA/g tannins. In order to determine the antioxidant capacity of the extract, we studied its 1,1-diphenyl-2-picryl-hydrazyl radicals (DPPH.) and reducing power activity assays. These colorimetric assays showed that PPME has strong reducing power and anti-radical capacity against DPPH with the IC50 values 0.210±0.005 mg/mL and 0.3±0.0045 mg/mL, respectively. Our findings clearly show that the methanolic extract of PPME contains two potent compounds against gastric ulcers compared to the reference drug, esomeprazole.
Journals System - logo
Scroll to top