Soil organic carbon (SOC) is an important component of the global carbon pool, whose accumulation and decomposition affect the balance of the global carbon cycle and climate change. However, understanding of the responses of SOC in alpine ecosystems to climate change is quite limited, especially in the seasonal-frozen soil zone. In order to understand the impact of soil moisture changes on the carbon cycle in alpine mountain ecosystems, we established two sample plots of the semishady and semi-sunny slopes in the subalpine meadow zone of the eastern part of Qilian Mountains, and collected soil samples to investigate the spatial and temporal changes of SOC and soil moisture and their relationships. The study indicated that the SOC content showed significant surface aggregation during the plant growing season, and that the influence of soil moisture on SOC was different in different slope directions and soil depths. The influence of soil moisture on SOC was greater on the semi-shady slope than on the semi-sunny slope. Among different soil layer depths, the soil moisture most significantly affected SOC in the soil layer of 20-40 cm. This study provides a theoretical basis for the study of carbon stocks and carbon cycling at high altitudes.