The purpose of this study was to identify the most important analgesic, anti-inflammatory, anxiolytic, antidepressant, antioxidant and anti-bacterial effects of Thymus vulgaris L. crop extracts obtained after optimization of extract time, temperature and speed conditions. Thyme crop extraction was established through magnetic stirring (MST) using ethanol after optimization of conditions using response surface methodology (RSM). Total phenols content (TPC), ferric reducing antioxidant power (FRAP), and scavenging activity (DPPH) were evaluated and then animals were subjected to Thyme extracts used for neuropharmacological and Antinociceptive properties determination by different experiments. DPPH, FRAP and TPC of Thyme leaves were about 3652.69 mg TE/100g DW; 5835.96 mg TE/100g DW and 1243.73 mg GAE/100g DW, respectively, for a temperature extraction (60ºC), time extraction (15 min) and speed extraction (600 rpm). The predicted values of DPPH, FRAP and TPC represent 92%, 89% and 90%, respectively compared with experiment values. Extraction temperature, extraction time and extraction speed affect positively on the DPPH, FRAP and TPC levels (p<0.05). Animals treated with Thyme extract (250 mg/kg) presented potential neuropharmacological and antinociceptive effects varied according to time of exposure. The promising neuropharmacological and antinociceptive effects obtained from Thyme extracts can be enhanced. The effects of the plant extracts analyzed vary widely not only from the plant species but also especially from the extraction conditions. The neuropharmacological and antinociceptive effects depend significantly on the treatment time.