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ORIGINAL RESEARCH
Antioxidant Activity of Plant Extracts
and the Enhance of Their Cytotoxic Effects
in Combination with Cisplatin
on HeLa Cell Line
1
Laboratory of Immunology and Toxicology, Faculty of Chemistry, Autonomous University of Coahuila;
Venustiano Carranza Blvd. and José Cárdenas Valdés Street, 25280, Saltillo, Coahuila, Mexico
2
Department of Food Research, Faculty of Chemistry, Autonomous University of Coahuila;
Venustiano Carranza Blvd. and José Cárdenas Valdés Street, 25280, Saltillo, Coahuila, Mexico
3
Department of Biochemistry and Pharmacology, Faculty of Chemistry, Autonomous University of Coahuila;
Venustiano Carranza Blvd. and José Cárdenas Valdés Street, 25280, Saltillo, Coahuila, Mexico
Submission date: 2023-11-28
Final revision date: 2024-01-20
Acceptance date: 2024-02-25
Online publication date: 2024-09-03
Publication date: 2025-01-28
Corresponding author
Crystel Aleyvick Sierra Rivera
Laboratory of Immunology and Toxicology, Autonomous University of Coahuila, José Cárdenas Valdés, 25280, Saltillo, Mexico
Laboratory of Immunology and Toxicology, Autonomous University of Coahuila, José Cárdenas Valdés, 25280, Saltillo, Mexico
Pol. J. Environ. Stud. 2025;34(3):2233-2251
KEYWORDS
TOPICS
ABSTRACT
In this study, the aqueous extracts from selected medicinal plants were evaluated for their
phytochemical composition, antitumor activity, and capacity to enhance the cytotoxic effects in
combination with cisplatin on human cervical cancer cells (HeLa). In addition, their antioxidant
properties were determined. The quantification of phenolic compounds and flavonoids on
the extracts was performed by the Folin-Ciocalteu and aluminum chloride methods, respectively.
The identification of phenolic compounds was determined by RP-HPLC-MS analysis. The evaluation
of the cytotoxic effects of the extracts, cisplatin, and their combinations on HeLa cells was performed
by MTT assay. The antioxidant activity was evaluated with the methods of DPPH, ABTS, and FRAP.
According to the results, the extracts showed the presence of phenolic compounds and flavonoids; the
identified metabolites mainly belonged to flavonols and hydroxycinnamic acids. Moreover, the extracts
decreased the viability of HeLa cells, with the extracts from Artemisia ludoviciana and Parthenium
hysterophorus with major cytotoxic activities. On the other hand, most of the combinations of
the extracts with cisplatin enhanced the cytotoxic effects on HeLa cells, being the combination with
the extract from Parthenium hysterophorus exhibiting the lowest percentage of cell viability. In addition,
the extracts showed antioxidant potential, with the extracts from Mentha piperita, Origanum vulgare, and Tilia mexicana having the most significant antioxidant effects. The aqueous extracts demonstrated
to be sources of phenolic compounds and flavonoids with cytotoxic activity on the HeLa cell line
and the capacity to enhance the cytotoxic effects in combination with cisplatin. Moreover, the plant
extracts showed antioxidant activity.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
REFERENCES (75)
1.
MARTÍNEZ-REYES I., CHANDEL N.S. Cancer metabolism: looking forward. Nature Reviews Cancer, 21 (1), 669, 2021. https://doi.org/10.1038/s41568... PMid:34272515 PMCid:PMC12826226.
2.
World Cancer Research Fund International. Available online: https://www.wcrf.org/cancer-tr... (accessed on 22nd July 2023).
3.
World Cancer Research Fund International. Available online: https://www.wcrf.org/cancer-tr... (accessed on 22nd July 2023).
4.
World Health Organization. Available online: https://www.who.int/news-room/... (accessed on 22nd July 2023).
5.
ZAHRA K., PATEL S., DEY T., PANDEY U., PRATAPMISHRA S. A study of oxidative stress in cervical cancer-an institutional study. Biochemistry and Biophysics Reports, 25 (1), 1, 2021. https://doi.org/10.1016/j.bbre... PMid:33437881 PMCid:PMC7786028.
6.
GHOSH S. Cisplatin: the first metal based anticancer drug. Bioorganic Chemistry, 88 (1), 1, 2019. https://doi.org/10.1016/j.bioo... PMid:31003078 PMCid:PMC11885776.
7.
TCHOUNWOU P.B., DASARI S., NOUBISSI F.K., RAY P., KUMAR S. Advances in our understanding of the molecular mechanisms of action of cisplatin in cancer therapy. Journal of Experimental Pharmacology, 13 (1), 303, 2021. https://doi.org/10.2147/JEP.S2... PMid:33776489 PMCid:PMC7987268.
8.
KUSHWAH A.S., GUPTA M.K., SINGH R., BANERJEE M. Cytokine gene variants and treatment outcome of cisplatin-based concomitant chemoradiotherapy in cervical cancer. British Journal of Biomedical Science, 77 (2), 81, 2020. https://doi.org/10.1080/096748... PMid:31935340.
9.
ALDOSSARY S.A. Review on pharmacology of cisplatin: clinical use, toxicity, and mechanism of resistance of cisplatin. Biomedical and Pharmacology Journal, 12 (1), 7, 2019. https://doi.org/10.13005/bpj/1....
10.
GRASSELLLY C., DENIS M., BOURGUIGNON A., TALHI N., MATHE D., TOURETTE A., SERRE L., JORDHEIM L.P., MATERA E.L., DUMONTET C. The antitumor activity of combination of cytotoxic chemotherapy and immune checkpoint inhibitors is model-dependent. Frontiers in Immunology, 9 (1), 1, 2018. https://doi.org/10.3389/fimmu.... PMid:30356816 PMCid:PMC6190749.
11.
PINMAI K., CHUNLARATTHANABHORN S., NGAMKITIDECHAKU C., SOONTHORNCHAREON N., HAHNVAJANAWONG C. Synergistic growth inhibitory effects of Phyllanthus emblica and Terminalia bellerica extracts with conventional cytotoxic agents: Doxorubicin and cisplatin against human hepatocellular carcinoma and lung cancer cells. World Journal of Gastroenterology, 14 (10), 1491, 2008. https://doi.org/10.3748/wjg.14... PMid:18330936 PMCid:PMC2693740.
12.
ALFARO-JIMÉNEZ M.A., ZUGASTI-CRUZ A., SILVA-BELMARES S.Y., ASCACIO-VALDÉS J.A., SIERRA-RIVERA C.A. Phytochemical, and biological characterization of aqueous and ethanolic extracts of Parthenium hysterophorus. Pharmacognosy Journal, 13 (1), 1, 2021. https://doi.org/10.5530/pj.202....
13.
MOSUNOVA O., NAVARRO-MUÑOZ J.C., COLLEMARE J. The biosynthesis of fungal secondary metabolites: from fundamentals to biotechnological applications. Encyclopedia of Mycology, 2 (1), 458, 2020. https://doi.org/10.1016/B978-0....
14.
KHALIL R., ALI Q., HAFEEZ M.M., MALIK A. Phenolic acid profiling by RP-HPLC: Evaluation of antibacterial and anticancer activities of Conocarpus erectus plant extracts. Biological and Clinical Sciences Research Journal, 10 (1), 1, 2020. https://doi.org/10.54112/bcsrj....
15.
SOLÍS-SALAS L.M., SIERRA-RIVERA C.A., COBOSPUC L.E., ASCACIO-VALDÉS J.A., SILVA-BELMARES S.Y. Antibacterial potential by rupture membrane and antioxidant capacity of purified phenolic fractions of Persea americana leaf extract. Antibiotics, 10 (5), 1, 2021. https://doi.org/10.3390/antibi... PMid:33946930 PMCid:PMC8146011.
16.
CRUZ-PÉREZ A.L., BARRERA-RAMOS J., BERNAL-RAMÍREZ L.A., BRAVO-AVILEZ D., RENDÓN-AGUILAR B. Actualized inventory of medicinal plants used in traditional medicine in Oaxaca, Mexico. Journal of Ethnobiology and Ethnomedicine, 17 (7), 1, 2021. https://doi.org/10.1186/s13002... PMid:33568185 PMCid:PMC7874459.
17.
GU C., HOWELL K., DUNSHEA F.R., SULERIA H.A.R. LC-ESI-QTOF/MS Characterization of phenolic acids and flavonoids in polyphenol-rich fruits and vegetables and their potential antioxidant activities. Antioxidants, 8 (9), 1, 2019. https://doi.org/10.3390/antiox... PMid:31533286 PMCid:PMC6770053.
18.
IQBAL J., KHAN A.A., AZIZ T., ALI W., AHMAD S., RAHMAN S.U., IQBAL Z., DABLOOL A.S., ALRUNWAYS M.W., ALMALKI A.A., ALMARI A.S., ALHOMRANI M. Phytochemical investigation, antioxidant properties and In vivo evaluation of the toxic effects of Parthenium hysterophorus. Molecules, 27 (1), 1, 2022. https://doi.org/10.3390/molecu... PMid:35807432 PMCid:PMC9268705.
19.
DE LEÓN-MEDINA J.C., SEPÚLVEDA-TORRE L., MORLETT-CHÁVEZ J., MELÉNDEZ-RENTEIRA P., ZUGASTI-CRUZ A., ASCACIO-VALDÉS J., AGUILAR C.N. Solid-state fermentation with Aspergillus niger GH1 to enhance polyphenolic content and antioxidative activity of Castilla Rose (Purshia plicata). Plants, 9 (11), 1, 2020. https://doi.org/10.3390/plants... PMid:33182299 PMCid:PMC7695294.
20.
ZUGASTI A., RIVERA A.L., SILVA S.Y., ALFARO M.A., SIERRA C.A. Effect of sodium dichloroacetate as single agent or in combination with cisplatin in normal and human cervical cell lines. Tropical Journal of Pharmaceutical Research, 19 (3), 467, 2020. https://doi.org/10.4314/tjpr.v....
21.
WONG-PAZ J.E., CONTRERAS-ESQUIVEL J.C., RODRÍGUEZ-HERRERA R., CARRILLO-INUNGARAY M.L., LÓPEZ L.I., NEVÁREZ-MOORILLÓN G.V., AGUILAR C.N. Total phenolic content, in vitro antioxidant activity and chemical composition of plant extracts from semiarid Mexican region. Asian Pacific Journal of Tropical Medicine, 8 (2), 104, 2015. https://doi.org/10.1016/S1995-... PMid:25902023.
22.
ARANDA-LEDESMA N.E., GOZÁLEZ-HERNÁNDEZ M.D., ROJAS R., PAZ-GONZÁLEZ A.S., RIVERA G., LUNA-SOSA B., MARTÍNEZ-ÁVILA G.C.G. Essential oil, and polyphenolic compounds of Fluorensia cernua leaves: chemical profiling and functional properties. Agronomy, 12 (10), 1, 2022. https://doi.org/10.3390/agrono....
23.
HERNÁNDEZ-MARÍN D.A., GUEVARA-LARA F., RIVAS-MORALES C., VERDUZCO-MARTÍNEZ J.A., GALINDO-RODRÍGUEZ S.A., SÁNCHEZ-GARCÍA E. Biological activity of Nothoscordum bivalve (L.) Britton and Parthenium incanum Kunth extracts. Indian Journal of Traditional Knowledge, 17 (4), 699, 2018.
24.
VIJAYASEKHAR V.E., PRASAD M.S., SUMAN JOSHI D.S.D., NARENDRA K., KRISHNA-SATYA A., SAMBASIVA-RAO K. R. Assessment of phytochemical evaluation and in-vitro antimicrobial activity of Cassia angustifolia. International Journal of Pharmacognosy and Phytochemical Research, 8 (2), 305, 2016.
25.
GUEMIDI C., SAADA D.A., CHABANE O.A., ELMASTAS M. Antioxidant potential, lipid profile and sensory attributes of a functional yogurt formulated with hydroethanolic extract of Mentha piperita L. Research Square, 1 (1), 1, 2023. https://doi.org/10.21203/rs.3.....
26.
GASPAR A., CRACIUNESCU O., TRIF M., MOISEI M., MOLDOVAN L. Antioxidant and anti-inflammatory properties of active compounds from Arnica montana L. Romanian Biotechnological Letters, 19 (3), 9353, 2014.
27.
OYEDEMI S.O., COOPOOSAMY R.M. Preliminary studies on the antibacterial and antioxidative potentials of hydroalcoholic extract from the whole parts of Artemisia vulgaris L. International Journal of Pharmacology, 11 (6), 561, 2015. https://doi.org/10.3923/ijp.20....
28.
HABEBALLA R.S., AHMEDANI E.I., AWAD N.S., ABDEIN M.A. In vitro antiviral activity of Illicium verum and Zingiber officinale ethanolic extracts. Medical Science, 24 (105), 3469, 2020.
29.
LIN D., XIAO M., ZHAO J., LI Z., XING B., LI X., KONG M., LI L., ZHANG Q., LIU Y., CHEN H., QIN W., WU H., CHEN S. An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 Diabetes. Molecules, 21 (10), 1, 2016. https://doi.org/10.3390/molecu... PMid:27754463 PMCid:PMC6274266.
30.
MORANDI-VUOLO M., SILVA-LIMA V., MARÓSTICA-JUNIOR M.R. Phenolic compounds: structure, classification, and antioxidant power. In book Bioactive compounds: Health benefits and potential applications, 1st ed.; Segura-Campos, M.R., Eds.; Woodhead Publishing, U.S.A., 1, pp. 33, 2019. https://doi.org/10.1016/B978-0....
31.
SOVA M., SASO L. Natural sources, pharmacokinetics, biological, activities and health benefits of hydroxycinnamic acids and their metabolites. Nutrients, 12 (8), 1, 2020. https://doi.org/10.3390/nu1208... PMid:32717940 PMCid:PMC7468728.
32.
BARRECA D., TROMBETTA D., SMERIGLIO A., MANDALARI G., ROMEO O., FELICE M.A., GATTUSO G., NABAVI S.M. Food flavonols: Nutraceuticals with complex health benefits and functionalities. Trends in Food Science and Technology, 117 (1), 194, 2021. https://doi.org/10.1016/j.tifs....
33.
WENDLOCHA D., KRZYKAWSKI K., MIELCZAREK-PALACZ A., KUBINA R. Selected flavonols in breast and gynecological cancer: A systematic review. Nutrients, 15 (3), 1, 2023. https://doi.org/10.3390/nu1513... PMid:37447264 PMCid:PMC10346890.
34.
WANG Z.Y., YIN Y., LI D.N., ZHAO D.Y., HUANG J.Q. Biological activities of p-hydroxycinnamic acids in maintaining gut barrier integrity and function. Foods, 12 (13), 1, 2023. https://doi.org/10.3390/foods1... PMid:37444374 PMCid:PMC10340781.
35.
KANIMOZHI G., PRASAD N.R. Anticancer effect of caffeic acid on human cervical cancer cells. In book Coffee in health and disease prevention., 1st ed.; Preedy, V.R., Eds.; Academic Press: U.S.A., 1, pp. 655, 2015. https://doi.org/10.1016/B978-0....
36.
MOHAMMED H.A., ABDULKARIN A.K., ALAMAMI A.D., ELSHIBANI F.A. Phytochemical constituents and biological activities of Jasonia glutinosa L.: The first report for the plant growing in North Africa. Journal of Chemistry, 2022 (1), 1, 2022. https://doi.org/10.1155/2022/4....
37.
KIM H.H., KIM J.K., KIM J., JUNG S.H., LEE K. Characterization of caffeoylquinic acids from Lepisorus thunbergianus and their melanogenesis inhibitory activity. ACS Omega, 5 (48), 30946, 2020. https://doi.org/10.1021/acsome... PMid:33324802 PMCid:PMC7726789.
38.
MEILAWATI L., DEWI R.M., TASFIYATI A.N., SEPTAMA A.W., ANTIKA L.D. Scopoletin: anticancer potential and mechanism of action. Asian Pacific Journal of Tropical Biomedicine, 13 (1), 1, 2023. https://doi.org/10.4103/2221-1....
39.
TIAN Q., WANG L., SUN X., ZENG F., PAN Q., XUE M. Scopoletin exerts anticancer effects on human cervical cancer cell lines by triggering apoptosis, cell cycle arrest, inhibition of cell invasion and PI3K/AKT signalling pathway. Journal of BUON, 24 (3), 997, 2019.
40.
KUETE V., KARAOSMANOĞLU O. Anticancer activities of african medicinal spices and vegetables. In book: Medicinal spices and vegetables from Africa, 1st ed.; Kuete, V.; Academic Press: U.S.A., 1, 271, 2017. https://doi.org/10.1016/B978-0....
41.
KHAN M., KHAN M., AL-HAMOUD K., ADIL S.F., SHAIK M.R., ALKHATHLAN H.Z. Comprehensive phytochemical analysis of various solvent extracts of Artemisia judaica and their potential anticancer and antimicrobial activities. Life, 12 (11), 1, 2022. https://doi.org/10.3390/life12... PMid:36431020 PMCid:PMC9697586.
42.
AKROUT A., ALARCON-GONZÁLEZ L., JANI H.E., CAMPRA-MADRID P. Antioxidant, and antitumor activities of Artemisia campestris and Thymelaea hirsuta from southern Tunisia. Food and Chemical Toxicology, 49 (2), 342, 2011. https://doi.org/10.1016/j.fct.... PMid:21075159.
43.
KIM J., JUNG K.H., YAN H.H., CHEON M.J., KANG S., JIN X., PARK S., OH M.S., HONG S.S. Artemisia capillaris leaves inhibit cell proliferation and induce apoptosis in hepatocellular carcinoma. BMC Complementary and Alternative Medicine, 18 (147), 1, 2018. https://doi.org/10.1186/s12906... PMid:29739391 PMCid:PMC5941330.
44.
SHARMA N., KUMAR M., TIWARI U., KALAKOTI M. The study of antimicrobial, anthelmintic, and cytotoxic activities of Parthenium hysterophorus L. International Journal of Engineering and Applied Sciences, 2 (10), 41, 2015.
45.
KUMAR S., PANDEY S., PANDEY A.K. In vitro antibacterial, antioxidant, and cytotoxic activities of Parthenium hysterophorus and characterization of extracts by LC-MS analysis. Biomed Research International, 2014 (1), 1, 2014. https://doi.org/10.1155/2014/4... PMid:24895583 PMCid:PMC4033558.
46.
ABBASZADEH H., KEIKHAEI B., MOTTAGHI S. A review of molecular mechanisms involved in anticancer and antiangiogenic effects of natural polyphenolic compounds. Phytotherapy Research, 33 (8), 2002, 2019. https://doi.org/10.1002/ptr.64....
47.
SHEN D.W., POULIOT L.M., HALL M.D., GOTTESMAN M.M. Cisplatin resistance: A cellular self-defense mechanism resulting from multiple epigenetic and genetic changes. Pharmacological Reviews, 64 (3), 706, 2012. https://doi.org/10.1124/pr.111... PMid:22659329 PMCid:PMC3400836.
48.
DASARI S., NJIKI S., MBEMI A., YEDJOU C.G., TCHOUNWOU P.B. Pharmacological effects of cisplatin combination with natural products in cancer chemotherapy. International Journal of Molecular Sciences, 23 (3), 1, 2022. https://doi.org/10.3390/ijms23... PMid:35163459 PMCid:PMC8835907.
49.
SIDHU H., CAPALASH N. Synergistic anti-cancer action of salicylic acid and cisplatin on HeLa cells elucidated by network pharmacology and in vitro analysis. Life Sciences, 282 (1), 1, 2021. https://doi.org/10.1016/j.lfs.... PMid:34237314.
50.
JEYA-SHREE T., GOWRI-SREE V., POOMPAVAI S., SGARBOSSA P., CAMARILLO I., SUNDARARAJAN R. Inhibition of proliferation of HeLa cells by pulsed electric field treated Mentha piperita (Mint) extract. IETE Journal of Research, 68 (2), 858, 2022. https://doi.org/10.1080/037720....
51.
CETOJEVIC-SIMIN D., CANADANOVIC-BRUNET J.M., BOGDANOVIC G.M., DJILAS S.M., CETKOVIC G.S., TUMBAS V.T., STOJILJKOVIC B.T. Antioxidative and antiproliferative activities of different horsetail (Equisetum arvense L.) extracts. Journal of Medicinal Food, 13 (2), 452, 2010. https://doi.org/10.1089/jmf.20... PMid:20170379.
52.
KOLDAS S., DEMIRTAS I., OZEN T., DEMIRCI M.A., BEHCET L. Phytochemical screening, anticancer and antioxidant activities of Origanum vulgare L. ssp. viride (Boiss.) Hayec, a plant of traditional usage. Journal of the Science of Food Agriculture, 95 (4), 786, 2015. https://doi.org/10.1002/jsfa.6... PMid:25200133.
53.
ABUBAKAR A.R., HAQUE M. Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes. Journal of Pharmacy and Bioallied Sciences, 12 (1), 1, 2020. https://doi.org/10.4103/jpbs.J... PMid:32801594 PMCid:PMC7398001.
54.
GHAVAMI G., MUHAMMADNEJAD S., AMANPOUR S., SARDARI S. Bioactivity screening of mulberry leaf extracts and two related flavonoids in combination with cisplatin on human gastric adenocarcinoma cells. Iranian Journal of Pharmaceutical Research, 19 (2), 371, 2020.
55.
SURESHKUMAR A., SENAWONG G., UTAIWAT S., PROMPIPAK J., WORANAM K., PHAOSIRI C., SRIPA B., SENAWONG T. Tiliacora triandra leaf powder ethanolic extract in combination with cisplatin or gemcitabine synergistically inhibits the growth of cholangiocarcinoma cells in vitro and in nude mouse xenograft models. Medicina, 59 (7), 1, 2023. https://doi.org/10.3390/medici... PMid:37512080 PMCid:PMC10386122.
56.
LUO H., DADDYSMAN M.K., RANKIN G.O., JIANG B.H., CHEN Y.C. Kaempferol enhances cisplatin's effect on ovarian cancer cells through promoting apoptosis caused by down regulation of cMyc. Cancer Cell International, 10 (16), 1, 2010. https://doi.org/10.1186/1475-2... PMCid:PMC2885998.
57.
ZHANG B.Y., WANG Y.M., GONG H., ZHAO H., LV X.Y., YUAN G.H., HAN S.R. Isorhamnetin flavonoid synergistically enhances the anticancer activity and apoptosis induction by cisplatin and carboplatin in nonsmall cell lung carcinoma (NSCLC). International Journal of Clinical and Experimental Pathology, 8 (1), 25, 2015.
58.
ORŠOLIC N., ODEH D., JAZVINISCAK-JEMBREK M., KNEŽEVIC J., KUCAN D. Interactions between cisplatin and quercetin at physiological and hyperthermic conditions on cancer cells in vitro and in vivo. Molecules, 25 (14), 1, 2020. https://doi.org/10.3390/molecu... PMCid:PMC7397216.
59.
PAPACHRISTOU F., ANNINOU N., KOUKOULIS G., PARASKAKIS S., SERTARIDOU E., TSALIKIDIS C., PITIAKOUDIS M., SIMOPOULOS C., TSAROUCHA A. Differential effects of cisplatin combined with the flavonoid apigenin on HepG2, Hep3B, and Huh7 liver cancer cell lines. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 866 (1), 1, 2021. https://doi.org/10.1016/j.mrge....
60.
MUTALIB N.A.B.D., MOHAMAD-BAKRI Z., ABDULJALIL K., LATIP N.A.B.D. Combination treatment of Chromolaena odorata ethanolic extract with cisplatin against breast cancer cell lines MDA-MB-231 and MCF-7. Journal of Medicinal Pharmaceutical and Allied Sciences, 12 (2), 5740, 2023.
61.
MAMAT N., ABDULLAH H., HAPIDIN H., MOKHTAR N.F. Gallic acid and methyl gallate enhance antiproliferative effect of cisplatin on cervical cancer (HeLa) cells. Sains Malaysiana, 49 (5) 1107, 2020. https://doi.org/10.17576/jsm-2....
62.
LIU F.T., AGRAWAL S.G., MOVASAGHI Z., WYATT P.B., REHMAN I.U., GRIBBEN J.G., NEWLAND A.C., JIA L. Dietary flavonoids inhibit the anticancer effects of the proteasome inhibitor bortezomib. Blood, 112 (9), 3835, 2008. https://doi.org/10.1182/blood-... PMCid:PMC5292595.
63.
WRÓBLEWSKA-ŁUCZKA P., GRABARSKA A., FLOREK-ŁUSZCZKI M., PLEWA Z., ŁUSZCZKI J.J. Synergy, additivity, and antagonism between cisplatin and selected coumarins in human melanoma cells. International Journal of Molecular Sciences, 22 (2), 1, 2021. https://doi.org/10.3390/ijms22... PMid:33430369 PMCid:PMC7827586.
64.
GULCIN I., ALWASEL S.H. DPPH radical scavenging assay. Processes, 11 (8), 1, 2023. https://doi.org/10.3390/pr1108....
65.
YADAV V.K., SINGH A.P. Antioxidant activity of the aqueous and ethanol extract of Mentha and guggul plant by DPPH method. International Journal of Health Sciences, 6 (S3), 4040, 2022. https://doi.org/10.53730/ijhs.....
66.
SURESHKUMAR J., AMALRAJ S., MURUGAN R., TAMILSELVAN A., KRUPA J., SRIRAMAVARATHARAJAN V., GURAV S.S., AYYANAR M. Chemical profiling and antioxidant activity of Equisetum ramosissimum Desf. Stem extract, a potential traditional medicinal plant for urinary tract infections. Future Journal of Pharmaceutical Sciences, 7 (192), 1, 2021. https://doi.org/10.1186/s43094....
67.
ABID K.Y., ABACHI F.T. Phytochemical comparative studies, antioxidant and antimicrobial of Artemisia and Star anise. Pharmacognosy Journal, 15 (1), 183, 2023. https://doi.org/10.5530/pj.202....
68.
ROMULO A. The principle of some in vitro antioxidant activity methods: review. IOP Conference Series Earth and Environmental Science, 426 (1), 012177, 2020. https://doi.org/10.1088/1755-1....
69.
BENSLAMA A., DACI S., ZAKARIA-NABTI L., BENDIF H., HARRAR A. Assessment of polyphenols contents, antibacterial and antioxidant activities of Origanum majorana extracts. European Journal of Biological Research, 11 (4), 509, 2021.
70.
JURIC T., MICIC N., POTKONJAK A., MILANOV D., DODIC J., TRIVUNOVIC Z., POPOVIC B.M. The evaluation of phenolic content, in vitro antioxidant, and antibacterial activity of Mentha piperita extracts obtained by natural deep eutectic solvents. Food Chemistry, 362 (1), 1, 2021. https://doi.org/10.1016/j.food... PMid:34118512.
71.
ZHELEVA-DIMITROVA D., BALABANOVA V. Antioxidant and acetylcholinesterase inhibitory potential of Arnica montana cultivated in Bulgaria. Turkish Journal of Biology, 36 (6), 732, 2012. https://doi.org/10.3906/biy-12....
72.
AYAZ-SEYHAN S., YILMAZ Z., CIKLA-YILMAZ D. The influence of extraction method on antioxidant potential of Tilia argentea flowers and bracts. Sciendo, 31 (1), 14, 2020. https://doi.org/10.2478/auoc-2....
73.
MICHALAKI A., KARANTONIS H.C., KRITIKOU A.S., THOMAIDIS N.S. Ultrasound-assisted extraction of total phenolic compounds and antioxidant activity evaluation from Oregano (Origanum vulgare ssp. hirtum) using response surface methodology and identification of specific phenolic compounds with HPLC-PDA and Q-TOF-MS/MS. Molecules, 28 (1), 1, 2023. https://doi.org/10.3390/molecu... PMid:36903279 PMCid:PMC10004109.
74.
ZEB A. Concept, mechanism, and applications of phenolic antioxidants in foods. Journal of Food Biochemistry, 44 (1), 1, 2020. https://doi.org/10.1111/jfbc.1... PMid:32691460.
75.
OLSZOWY M. What is responsible for antioxidant properties of polyphenolic compounds from plants? Plant Physiology and Biochemistry, 144 (1), 135, 2019. https://doi.org/10.1016/j.plap... PMid:31563754.
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| eISSN: | 2083-5906 |
| ISSN: | 1230-1485 |
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