ORIGINAL RESEARCH
Assessment of Wheat Biofortification
for Iron (Fe) Using Vermicompst
and Biochar along with Fe Application
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1
Department of Soil and Environmental Sciences, Ghazi University Dera Ghazi Khan, Pakistan
2
Department of Plant Protection, Ghazi University, Pakistan
3
Department of Horticulture, Ghazi University, Pakistan
4
Department of Botany, Sargodha University, Pakistan
5
Department of Biotechnology, College of Science, Taif University, Taif City, Saudi Arabia
6
Department of Chemistry, College of Science, Taif University, Saudi Arabia
These authors had equal contribution to this work
Submission date: 2024-04-11
Final revision date: 2024-06-27
Acceptance date: 2024-09-09
Online publication date: 2024-12-20
Publication date: 2025-11-04
Corresponding author
Saqib Bashir
Department of Soil and Environmental Sciences, Ghazi University Dera Ghazi Khan, Pakistan
Pol. J. Environ. Stud. 2025;34(6):7265-7271
KEYWORDS
TOPICS
ABSTRACT
Iron-biofortification is a sustainable method of addressing iron (Fe) deficiency in crops by increasing
iron content and bioavailability under alkaline conditions. In this regard, the management of Fe-deficient
soil is becoming a serious matter of concern for soil health and the food web. The current research was
carried out to investigate the possible impact of vermicompost (VC) and biochar (BC) along with iron
(Fe) for improving the Fe fortification in wheat (Triticum aestivum L.) grown in alkaline soil. The wheat
genotype “Fakher-e-Bakkhr” was used as a test plant. The findings showed the highest variations
among all the measured parameters. The use of Fe along with VC and BC significantly improved plant
height, root length, root and shoot weight, number of leaves, and overall plant biomass of wheat crop.
A greater increment was noticed in available Fe in soil when VC, BC, and VC+BC along with Fe were
applied in alkaline soil by 31.4%, 36.4%, and 41.5%, respectively, over control. Additionally, Fe contents
in plants were increased by 16.5%, 22.6%, and 25.1% when VC, BC, and VC+BC along with Fe were
applied in alkaline soil. Similarly, maximum increases in soil OM, available P, and K by 53%, 21.9%,
and 19% were recorded when VC+BC+Fe was added in combined form relative to control. This study
indicated that using organic amendments like BC and VC along with iron can have the potential to
improve the bioavailability of iron and enhance wheat nutritional quality, thereby enhancing the Fe
fortification in wheat under alkaline conditions.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
REFERENCES (33)
1.
HAFEEZ M.B., RAMZAN Y., KHAN S., IBRAR D., BASHIR S., ZAHRA N., RASHID N., NADEEM M., RAHMAN S., SHAIR H. Application of zinc and iron-based fertilizers improves the growth attributes, productivity, and grain quality of two wheat (Triticum aestivum) cultivars. Frontier in Nutrition, 8, 1036, 2021.
https://doi.org/10.3389/fnut.2....
2.
CAKMAK I., KUTMAN U.B. Agronomic biofortification of cereals with zinc: A review. European Journal of Soil Science, 69, 172, 2018.
https://doi.org/10.1111/ejss.1....
3.
GHESHLAGHI Z.M., PASARI B., SHAMS K., ROKHZADI A., MOHAMMADI K. The effect of micronutrient foliar application on yield, seed quality and some biochemical traits of soybean cultivars under drought stress. Journal of Plant Nutrition, 42, 2715, 2019.
https://doi.org/10.1080/019041....
4.
KLIKOCKA H., MARKS M. Sulphur and nitrogen fertilization as a potential means of agronomic biofortification to improve the content and uptake of microelements in spring wheat grain DM. Journal of Chemistry, 2018 (2), 1, 2018.
https://doi.org/10.1155/2018/9....
5.
ULLAH A., FAROOQ M., REHMAN A., ARSHAD M., SHOUKAT H., NADEEM A., NAWAZ A., WAKEEL A., NADEEM F. Manganese nutrition improves the productivity and grain biofortification of bread wheat in alkaline calcareous soil. Experimental Agriculture, 54, 744, 2018.
https://doi.org/10.1017/S00144....
6.
NAVEED M., RAMZAN N., MUSTAFA A., SAMAD A., NIAMAT B., YASEEN M., AHMAD Z., HASANUZZAMAN M., SUN N., SHI W., XU M. Alleviation of salinity induced oxidative stress in Chenopodium quinoa by Fe biofortification and biochar-endophyte interaction. Agronomy, 10 (2), 168, 2020.
https://doi.org/10.3390/agrono....
7.
TORRE-ROCHE D.L.R., CANTU J., TAMEZ C., ZUVERZA-MENA N., HAMDI H., ADISA I.O., ELMER W., TORRESDEY J.G., WHITE J.C. Seed biofortification by engineered nanomaterials: A pathway to alleviate malnutrition. Journal of Agriculture Food Chemistry, 68, 12189, 2020.
https://doi.org/10.1021/acs.ja....
8.
LIGOWE I.S., BAILEY E.H., YOUNG S.D., ANDER E.L., KABAMBE V., CHILIMBA A.D., LARK R.M., NALIVATA P.C. Agronomic iodine biofortification of leafy vegetables grown in Vertisols, Oxisols and Alfisols. Environmental Geochemistry Health, 43, 361, 2021.
https://doi.org/10.1007/s10653....
9.
FAKHARZADEH S., HAFIZI M., BAGHAEI M.A., ETESAMI M., KHAYAMZADEH M., KALANAKY S., AKBARI M.E., NAZARAN M.H. Using Nano-chelating Technology for Biofortification and Yield Increase in Rice. Scientific Report, 10, 4351, 2020.
https://doi.org/10.1038/s41598....
10.
TABAK M., LISOWSKA A., FILIPEK-MAZUR B., ANTONKIEWICZ J. The effect of amending soil with waste elemental sulfur on the availability of selected macroelements and heavy metals. Processes, 8 (10), 1245, 2020.
https://doi.org/10.3390/pr8101....
11.
ADEKIYA A.O., AGBEDE T.M., ABOYEJI C.M., DUNSIN O., SIMEON V.T. Effects of biochar and poultry manure on soil characteristics and the yield of radish. Scientia Horticulturae, 243, 457, 2019.
https://doi.org/10.1016/j.scie....
12.
GRABER E.R., TSECHANSKY L., LEW B., COHEN E. Reducing capacity of water extracts of biochars and their solubilization of soil Mn and Fe. European Journal of Soil Science, 65 (1), 162, 2014.
https://doi.org/10.1111/ejss.1....
13.
VYAS P., SHARMA S., GUPTA J. Vermicomposting with microbial amendment: Implications for bioremediation of industrial and agricultural waste. Bio Technologia, 103, 203, 2022.
https://doi.org/10.5114/bta.20....
14.
WANG S., GAO P., ZHANG Q., SHI Y., GUO X., LV Q. Biochar improves soil quality and wheat yield in saline-alkali soils beyond organic fertilizer in a 3-year field trial. Environmental Science and Pollution Research, 30, 19097, 2023.
https://doi.org/10.1007/s11356....
15.
BASHIR A., RIZWAN M., ALI S., ADREES M., REHMAN M.Z.U., QAYYUM M.F. Effect of composted organic amendments and zinc oxide nanoparticles on growth and cadmium accumulation by wheat; a life cycle study. Environmental Science and Pollution Research, 27, 23926, 2020.
https://doi.org/10.1007/s11356....
16.
WALKLEY A., BLACK I.A. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science, 37 (1), 29, 1934.
https://doi.org/10.1097/000106....
17.
OLSEN S.R. Estimation of available phosphorus in soils by extraction with sodium bicarbonate (No. 939). US Department of Agriculture, 1954.
18.
BASHIR S., HUSSAIN Q., SHAABAN M., HU H. Efficiency and surface characterization of different plant derived biochar for cadmium (Cd) mobility, bioaccessibility and bioavailability to Chinese cabbage in highly contaminated soil. Chemosphere, 211, 632, 2018.
https://doi.org/10.1016/j.chem....
19.
TASKIN M.B., GUNES A. Iron biofortification of wheat grains by foliar application of nano zero-valent iron (nZVI) and other iron sources with urea. Journal of Soil Science and Plant Nutrition, 22 (4), 4642, 2022.
https://doi.org/10.1007/s42729....
20.
OWAIDAH T., AL-NUMAIR N., AL-SULIMAN A., ZOLALY M., HASANATO R., AL ZAHRANI F., SAJID M.R. Iron deficiency and iron deficiency anemia are common epidemiological conditions in Saudi Arabia: report of the national epidemiological survey. Anemia, 2020, 1, 2020.
https://doi.org/10.1155/2020/6....
21.
SALEEM A., ZULFIQAR A., SALEEM M.Z., ALI B., SALEEM M.H., ALI S., TUFEKCI E.D., TUFEKCI A.R., RAHIMI M., MOSTAFA R.M. Alkaline and acidic soil constraints on iron accumulation by Rice cultivars in relation to several physio-biochemical parameters. BMC Plant Biology, 23 (1), 397, 2023.
https://doi.org/10.1186/s12870....
22.
AMANULLAH, INAMULLAH, ALWAHIBI M.S., ELSHIKH M.S., ALKAHTANI J., MUHAMMAD A., KHALID S., AHMAD I., KHAN N., ULLAH S. Phosphorus and zinc fertilization improve zinc biofortification in grains and straw of coarse vs. fine rice genotypes. Agronomy, 10 (8), 1155, 2020.
https://doi.org/10.3390/agrono....
23.
SABIJON J., SUDARIA M.A. Effect of vermicompost amendment and nitrogen levels on soil characteristics and growth and yield of tomato (Solanum lycopersicum cv. Diamante max). International Journal of Agriculture Forestry and Life Sciences, 2 (2), 145, 2018.
24.
LÓPEZ R., ANTELO J., SILVA A.C., BENTO F., FIOL S. Factors that affect physicochemical and acid-base properties of compost and vermicompost and its potential use as a soil amendment. Journal of Environmental Management, 300, 13702, 2021.
https://doi.org/10.1016/j.jenv....
25.
SARMA B., FAROOQ M., GOGOI N., BORKOTOKI B., KATAKI R., GARG A. Soil organic carbon dynamics in wheat-Green gram crop rotation amended with vermicompost and biochar in combination with inorganic fertilizers: A comparative study. Journal of Cleaner Production, 201, 471, 2018.
https://doi.org/10.1016/j.jcle....
26.
MANZOOR M.A.L., NI K., RUAN J. Effect of integrated use of rapeseed cake, biochar and chemical fertilizers on root growth, nutrients use efficiency and productivity of tea. Agronomy, 12, 1823, 2022.
https://doi.org/10.3390/agrono....
27.
WAN H., LIU X., SHI Q., CHEN Y., JIANG M., ZHANG J. Biochar amendment alters root morphology of maize plant: its implications in enhancing nutrient uptake and shoot growth under reduced irrigation regimes. Frontier in Plant Science, 14, 1122742, 2023.
https://doi.org/10.3389/fpls.2....
28.
ZUO Y., ZHANG J., ZHAO R., DAI H., ZHANG Z. Application of vermicompost improves strawberry growth and quality through increased photosynthesis rate, free radical scavenging and soil enzymatic activity. Scientia Horticulture, 233, 132, 2018.
https://doi.org/10.1016/j.scie....
29.
LIU X., ZHANG J., WANG Q., CHANG T., SHAGHALEH H., HAMOUD Y.A. Improvement of photosynthesis by biochar and vermicompost to enhance tomato (Solanum lycopersicum L.) yield under greenhouse conditions. Plants, 11 (23), 3214, 2022.
https://doi.org/10.3390/plants....
30.
RAMZAN Y., HAFEEZ M.B., KHAN S., NADEEM M., BATOOL S., AHMAD J. Biofortification with zinc and iron improves the grain quality and yield of wheat crop. International Journal of Plant Production, 14, 501, 2020.
https://doi.org/10.1007/s42106....
31.
ESTEFAN G., SOMMER R., RYAN J. Methods of soil, plant, and water analysis. A manual for the West Asia and North Africa region, 3, 65, 2013.
32.
NAUŠ J., PROKOPOVÁ J., ŘEBÍČEK J., ŠPUNDOVÁ M. SPAD chlorophyll meter reading can be pronouncedly affected by chloroplast movement. Photosynthesis Research, 105, 265, 2010.
https://doi.org/10.1007/s11120....
33.
GUPTA O.P., SINGH A., PANDEY V., SENDHIL R., KHAN M.K., PANDEY A., KUMAR S., HAMURCU M., RAM S., SINGH G. Critical assessment of wheat biofortification for iron and zinc: a comprehensive review of conceptualization, trends, approaches, bioavailability, health impact, and policy framework. Frontiers in Nutrition, 10, 1310020, 2024.
https://doi.org/10.3389/fnut.2....