Agroforestry is the integration of trees or shrubs with crops and/or livestock on the same plot and/or landscape. The Agroforestry System (AFS) is a promising agroecological approach for climate change adaptation and mitigation through carbon sequestration in both biomass and soil. This work aimed to assess the biomass of fig trees (Ficus carica) and their intercrop, their carbon stock (C stock) and CO₂ equivalent (CO₂-eq), as well as the soil organic carbon stock (SOC stock) and soil CO₂ equivalent (CO₂- eq) at a depth of 0-30 cm. The experimental work was conducted in the Djebba region, located in the northwest of Tunisia, during 2023 and 2024. This experimental site was called Djebba Living Lab. In the latter, 5 AFSs were selected based on the variability of intercrop, given that the main tree species was a landrace fig tree, “Bouhouli”. The intercrops which were sown under and very close to fig trees in AFS1, AFS3, AFS4, and AFS5 are zucchini, barley, faba bean, and pepper, respectively. AFS2 was a control system composed solely of fig trees in monoculture. In 2024, the highest biomass, C stock, and CO₂ equivalent were recorded in the trees of AFS4, with 78.59 t/ha, 36.94 t/ha, and 135.57 t/ha, respectively. The assessment of soil showed that SOC stock and CO₂ equivalent were 21.41 t/ha and 78.58 t/ha, respectively. AFS5 (mixture of market gardening and pepper landrace) outperformed all others, achieving the highest annual biomass accumulation (2.21 t ha⁻¹ yr⁻¹), C stock (1.03 t ha⁻¹ yr⁻¹), and CO₂-eq (3.81 t ha⁻¹ yr⁻¹). However, the monoculture system (AFS2) registered the lowest values with annual biomass accumulation (0.17 t/ha/year), C stock (0.08 t/ha/year), and CO₂-eq (0.30 t/ha/year). Agroforestry has been acknowledged as a climate change mitigation strategy. Through this agronomic practice, AFSs with various intercropping have the potential to increase the surface area through the use of understory crops, improve the biomass, and subsequently increase the carbon sequestration in plants and soil.