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eISSN: 2083-5906
ISSN: 1230-1485
ISSN: 1230-1485
ORIGINAL RESEARCH
From Methodological Foundations to Biodiversity
Applications: A Comprehensive Analysis of
Environmental DNA Research Trends (1992–2024)
1
College of Water Sciences, Beijing Normal University, Beijing 100875, China
2
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental
Sciences, Beijing 100012, China
3
College of Life Sciences and Technology, Tarim University, Alar 843300, China
4
College of Material Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China
5
College of Ecology, Lanzhou University, Lanzhou 730000, China
6
Command Center for Comprehensive Survey of Natural Resources, China Geological Survey Bureau, Beijing 100055,
China
Submission date: 2025-01-19
Final revision date: 2025-04-25
Acceptance date: 2025-05-17
Online publication date: 2025-08-21
Corresponding author
Jiang Chang
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
Junsheng Li
Command Center for Comprehensive Survey of Natural Resources, China Geological Survey Bureau, Beijing 100055, China
Command Center for Comprehensive Survey of Natural Resources, China Geological Survey Bureau, Beijing 100055, China
KEYWORDS
TOPICS
ABSTRACT
Over the last decade, environmental DNA (eDNA)-based bioassessment has emerged as one of the
most rapidly evolving and widely adopted methodologies in biodiversity research. In this context, we
present a comprehensive overview of the eDNA research field based on a bibliometric analysis of 4524
documents published between 1992 and 2024. The journals with the most published articles on eDNA
were Environmental DNA, PLOS ONE, and Molecular Ecology Resources. The countries with the most
eDNA publications were the United States, China, and Japan. The structural topic modeling method
employed allowed us to identify the main research topics, their interrelationships, and their temporal
dynamics. Based on the temporal change in research topics, the application of eDNA research has
shifted from methodological research to biodiversity and community research since 1992. The results
were interpreted to provide insights into the current state of the eDNA research field, shedding light on
the advancements, challenges, and potential applications of eDNA metabarcoding.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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