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ORIGINAL RESEARCH
Microbial Diversity in the Air of Chicken Coops
under Different Feeding Methods Based on
High-Throughput Sequencing
1
Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences,
Jinan 250100, China
2
Shandong Provincial Key Laboratory of Poultry Diseases Diagnosisand Immunology, Jinan 250023, China
3
Institute of Poultry Sciences, Shandong Academy of Agricultural Sciences, Jinan 250100, China
4
Yunnan Shennong Agricultural Industry Group Co., LTD, Yunnan 650000, China
These authors had equal contribution to this work
Submission date: 2024-06-25
Final revision date: 2024-09-09
Acceptance date: 2024-10-28
Online publication date: 2025-01-08
Publication date: 2025-11-14
Corresponding author
Lihong Qi
Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China
Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China
Jiaqiang Wu
Shandong Provincial Key Laboratory of Poultry Diseases Diagnosisand Immunology, Jinan 250023, China
Shandong Provincial Key Laboratory of Poultry Diseases Diagnosisand Immunology, Jinan 250023, China
Pol. J. Environ. Stud. 2025;34(6):8087-8095
KEYWORDS
TOPICS
ABSTRACT
Microbial aerosol particles carried in chicken coops are a major cause of respiratory diseases in both
poultry and workers. This study aimed to investigate the microbial community structure in microbial
aerosol particles under three different feeding methods and analyze the composition of the bacterial
community in the air of chicken coops using high-throughput sequencing. The results revealed that
the dominant phyla in all samples were Firmicutes, Proteus, Actinomyces, and Bacteroides. Among
these, Firmicutes had a higher proportion in the coop air. However, at the genus level, there were
significant differences in microbial species among the three rearing practices. Additionally, potential
pathogenic bacteria were found in the coop air, including Staphylococcus, Acinetobacter, Streptococcus,
Flavobacterium, Clostridium, Rhodococcus, and Campylobacter. In conclusion, the air in chicken coops
contains various types of small and medium-sized microorganisms, including some pathogenic bacteria
that can infect livestock, poultry, and breeding staff. It is recommended to utilize thick bedding feeding
and cage feeding methods while also strengthening cleaning and disinfection protocols in the chicken
coops.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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