ORIGINAL RESEARCH
The Effect of Organic Compost Dosage on Changes in Soil Dissolved Organic Matter Components Analyzed with Three-dimensional Fluorescence Spectroscopy and Ultraviolet Spectroscopy
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1
College of Agriculture and Forestry Science, Hebei North University, Zhangjiakou 075000, China
 
2
Hebei Forestry and Grassland Bureau Scientific Research Institute, Shijiazhuang 050000, China
 
3
Agriculture and Rural Bureau of Chicheng County, Zhangjiakou City, Zhangjiakou 075500, China
 
 
Submission date: 2025-02-12
 
 
Final revision date: 2025-10-15
 
 
Acceptance date: 2025-11-15
 
 
Online publication date: 2026-04-10
 
 
Corresponding author
Haichao Zhao   

College of Agriculture and Forestry Science, Hebei North University, Zhangjiakou 075000, China
 
 
Haixiang Zhao   

Hebei North University, Hebei Key Laboratory of Quality & Safety Analysis-Testing for Agro-Products and Food, China
 
 
 
KEYWORDS
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ABSTRACT
The application of organic compost in farmland is a major agronomic measure to increase soil organic matter content, enhance soil nutrients, and regulate soil microbial activity and other soil micro components. The influence of organic compost dosage on soil DOM components was studied. In this study, greenhouse pot experiments were conducted with 7 cattle manure organic compost dosage ratios of 0%, 5%, 10%, 20%, 40%, 60%, and 80%. Three-dimensional fluorescence spectroscopy with fluorescence regional integrated volume integration (3DEEM-FRI) and ultraviolet spectroscopy (UV) were used to analyze the effect of organic compost dosage on soil DOM components and structural characteristics. The results indicated that with the increase in organic compost dosage, the soil TOM content showed a linear increase, and the soil DOM content showed an exponential increase. The proportion of humic acid-like components in soil DOM showed a linearly increasing trend, and the proportion of tryptophan protein and fulvic acid components showed a linearly decreasing trend. The proportion of tyrosine protein and microbial metabolites, as well as lignin and quinine in DOM components, showed Exponential (Exp3P2) changes which first presented an increasing and then a decreasing trend, and reached a turning point at 20 g∙kg⁻¹ of TOM. With the increase in the soil TOM content, the substituents on the benzene ring of soil DOM transformed from non-polar functional groups, such as fatty chains and esters, to polar functional groups such as hydroxyl, carboxyl, and carbonyl. The molecular condensation degree, molecular weight, and humification degree of the soil DOM showed a linearly increasing trend. The content of the hydrophobic organic component and the aromaticity of the soil DOM showed a linearly decreasing trend, and the proportion of biogenic sources showed an exponentially decreasing trend. The proportion of non-humified and humified substances in the soil DOM showed an initial increase and then a decreasing trend and reached the highest value at 35 g∙kg-1 of TOM. The organic compost dosage affected the proportion and molecular structure of the soil DOM components by regulating the biological effects of microorganisms and enzyme activity, as well as the conversion process from soil TOM to DOM.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
REFERENCES (37)
1.
XIA M., LI P., LIU J., QIN W., DAI Q., WU M., LI Z., LI D., LIU M. Long-term fertilization promotes the microbialmediated transformation of soil dissolved organic matter. Communications Earth & Environment. 6, 114, 2025.
 
2.
ZHOU P., TIAN L., GRAHAM N., SONG S., ZHAO R., SIDDIQUE M.S., HU Y., CAO X., LU Y., ELIMELECH M., YU W. Spatial patterns and environmental functions of dissolved organic matter in grassland soils of China. Nature Communications. 15, 1, 2024.
 
3.
ZHOU P., TIAN L., SIDDIQUE M.S., SONG S., GRAHAM N.J.D., ZHU Y.G., YU W.Z. Divergent fate and roles of dissolved organic matter from spatially varied grassland soils in China during long-term biogeochemical processes. Environmental Science & Technology. 58 (2), 1164, 2024.
 
4.
PLAZA C., XING B., FERNÁNDEZ J.M., SENESI N., POLO A. Binding of polycyclic aromatic hydrocarbons by humic acids formed during composting. Environmental Pollution. 157 (1), 257, 2009.
 
5.
WU Y., JI H., WU W., WU Z., XIE Y., FU P. Size fractionation and optical properties of DOM under different land use types in the coastal area of northern Hainan Island. Journal of Agricultural Resources and Environment. 37 (5), 654, 2020 [In Chinese].
 
6.
ZHAO H.C., LIU Z.G., LU H.B., ZHAO H.X., HUANG Z.H., WEI D., LIU J.H. The effect mechanism of fertilization measures on the three-dimensional fluorescence characteristics of soil DOM components. Polish Journal of Environmental Studies. 31 (6), 5443, 2022.
 
7.
HE X.S., XI B.D., WEI Z.M., JIANG Y.H., YANG Y., AND., CAO J.L., LIU H.L. Fluorescence excitation-emission matrix spectroscopy with regional integration analysis for characterizing composition and transformation of dissolved organic matter in landfill leachates. Journal of Hazardous Materials. 190 (1-3), 293, 2011.
 
8.
LI Q., WEI Y., CUI H., FENG H., LANG J. Quantitative analysis of TOC in water quality based on UV-Vis spectroscopy. Spectroscopy and Spectral Analysis. 42 (2), 376, 2022 [In Chinese].
 
9.
ZHAO X., WU D., LI Q., WANG X., CHEN M. Response of dissolved organic matter chemical properties to Long-term different fertilization in latosol: Insight from Ultraviolrt-Visible spectroscopy. Spectroscopy and Spectral Analysis. 42 (10), 3210, 2022 [In Chinese].
 
10.
ZHAI T.N., HUO S.L., ZHANG J.T. Spectral characteristics of vertical distribution of dissolved organic matters in sediments. Chinese Journal of Environmental Engineering. 11 (11), 6196, 2017 [In Chinese].
 
11.
CHEN W., WESTERHOFF P., LEENHEER J.A., BOOKSH K. Fluorescence excitation- emission matrix regional integration to quantify spectra for dissolved organic matter. Environmental Science Technology. 37 (24), 5701, 2003.
 
12.
ZHANG L., WANG S.R., ZHAO H.C., LI Y.P., HUO S.L., QIAN W.B., YANG Y.L., CHENG J. Using multiple combined analytical techniques to characterize water extractable organic nitrogen from Lake Erhai sediment. Science of the Total Environment. 542, 344, 2016.
 
13.
HE J., YANG Y., WU X., ZHI G., ZHANG Y., SUN X., JIAO L., DENG W., ZHOU H., SHAO Z., ZHU Q. Responses of dissolved organic matter (DOM) characteristics in eutrophic lake to water diversion from external watershed. Environmental Pollution. 312, 119992, 2022.
 
14.
LIU F., CHEN L., ZHUANG W., YANG L. Effects of photochemical and microbial degradation on the interaction between dissolved organic matter released from reed leaf litter and lead. Acta Scientiae Circumstantiae. 42 (9), 113, 2022 [In Chinese].
 
15.
DERRIEN M., CHOI H., JARDÉ E., SHIN K.H., HUR J. Do early diagenetic processes affect the applicability of commonly-used organic matter source tracking tools? An assessment through controlled degradation end-member mixing experiments. Water Research. 173, 115588, 2020.
 
16.
LIU C., BAI X.D., ZHAO H.C., HUANG Z.H., LIU S.T., LU H.B., LIU Z.G., LIU X.L. The effect mechanism of spring maize straw returning method on soil DOM spectral characteristics in cold and arid regions of China. Ecology and Environmental Sciences. 32 (8), 1419, 2023 [In Chinese].
 
17.
DERRIEN M., YANG L., HUR J. Lipid biomarkers and spectroscopic indices for identifying organic matter sources in aquatic environments: A review. Water Research. 112, 58, 2017.
 
18.
WANG D., ZHOU H., ZUO J., CHEN P., SHE Y., YAO B., DONG S., WU J., LI F., NJOROGE D.M., SHI G., MAO X., MA L., ZHANG Z., MAO Z. Responses of soil microbial metabolic activity and community structure to different degraded and restored grassland gradients of the Tibetan plateau. Frontiers in Plant Science. 13, 770315, 2022.
 
19.
GE X.L., QIAN C.R., LI L., JIANG Y.B., GONG X.J., LV G.Y. Effects of straw returning cooperated with fertilizer practice on yield of maize and soil quality of tillage layer. Soil and Fertilizer Sciences in China. (1), 131, 2021 [In Chinese].
 
20.
WU H.W., CUI H.L., FU C.X., LI R., QI F.Y., LIU Z.L., YANG G., XIAO K.Q., QIAO M. Unveiling the crucial role of soil microorganisms in carbon cycling: A review. Science of The Total Environment. 909 (1), 168627, 2024.
 
21.
LIU M., ZHANG Z., HE Q., WANG H., LI X., SCHOER J. Exogenous phosphorus inputs alter complexity of soil-dissolved organic carbon in agricultural riparian wetland. Chemosphere. 95, 572, 2014.
 
22.
MARSCHNER B., KALBITZ K. Controls of bioavailability and biodegradability of dissolved organic matter in soils. Geoderma. 113 (3-4), 211, 2003.
 
23.
CHEN Q., CHEN J., CAI R., HE C., SHI Q., ZHANG C., JIAO N., ZHENG Q. Deciphering microbially driven labile and refractory molecular candidates in dissolved organic matter. Journal of Geophysical Research: Biogeosciences. 130 (2), JG008484, 2025.
 
24.
PAN H.W., TONG W.B., LEI H.J., YANG G., SHI L.L. Spectral Analysis of the effect of organic fertilizer application on the evolution of organic matter and nitrogen in farmland. Specteoscopy and Spectral Analysis. 42 (10), 3116, 2022 [In Chinese].
 
25.
AWASTHI M.K., WANG Q., HUANG H., REN X., LAHORI A.H., MAHAR A., ALI A., SHEN F., LI R., ZHANG Z. Influence of zeolite and lime as additives on greenhouse gas emissions and maturity evolution during sewage sludge composting. Bioresource Technology. 216, 172, 2016.
 
26.
PENG J., WANG K., GU Y., WANG A.J. Effects of environmental factors on tetracycline and macrolides resistance genes in cattle manure composting systems. Environmental Science. 40 (3), 1439, 2019 [In Chinese].
 
27.
WANG D.M., MAI L.W., YU Z., WANG K.T., MENG Z., WANG X.F., LI Q.F., LIN J.C., WU D.M. Deciphering the bioavailability of dissolved organic matter in thermophilic compost and vermicompost at the molecular level. Bioresource Technology. 391, 129947, 2024.
 
28.
TAO S., LIN B. Water soluble organic carbon and its measurement in soil and sediment. Water Research. 34 (5), 1751, 2000.
 
29.
QIAN H.Y., CHEN Z.Y., FENG X.X., ZHOU N.D., LUO Y.J., PU Y.L., HU Y.F. Content and spectral characteristics of soil dissolved organic matter in alpine meadows with different degrees. Acta Agrestia Sinica. 32 (12), 3715, 2024 [In Chinese].
 
30.
BIYADA S., MERZOUKI M., ELKARRACH K., BENLEMLIH M. Spectroscopic characterization of organic matter transformation during composting of textile solid waste using UV-Visible spectroscopy, infrared spectroscopy and X-ray diffraction (XRD). Microchemical Journal. 159, 105314, 2020.
 
31.
YANG X., BAO Y.W., LI B.W., WANG R.X., SUN C., MA D.H., CHEN L., ZOU H.T., ZHANG J.B. Effects of fertilization applications on soil aggregate organic carbon content and assessment of their influencing factors: A meta-analysis. Catena. 242, 108135, 2024.
 
32.
KALBITZ K., SCHMERWITZ J., SCHWENSIG D., MATZNER E. Biodegradation of soil-derived dissolved organic matter as related to its properties. Geoderma. 113 (3-4), 273, 2003.
 
33.
CAI S.J., ZHOU X., LIU Y., TONG Z.Y., XU C., QU L.Y., LI P.H., XIAO W.P., GUO W.D., HUANG B.Q. Shaping microbial communities in coastal systems through DOM transformation in river plumes. Water Research. 284, 123996, 2025.
 
34.
FU Y.Y., LIU W.L., CHEN Z.Y., REDMILE-GORDON M., LIANG C., TANG C.X., GUGGENBERGER G., YAN S.B., YIN L.M., PENG J.J., ZWIETEN L.V., WANG P., CHEN J., KUZYAKOV Y., GE T.D., XU J.M., LUO Y. Microbial metabolisms determine soil priming effect induced by organic inputs. Soil Biology and Biochemistry. 209, 109885, 2025.
 
35.
DAI T.J., WEN D.H., BATES C.T., WU L.W., GUO X., LIU S., SU Y.F., LEI J.S., ZHOU J.Z., YANG Y.F. Nutrient supply controls the linkage between species abundance and ecological interactions in marine bacterial communities. Nature Communications. 13, 175, 2022.
 
36.
HAN Y., ZHANG X., SHI Z., HAO M., WEI X., SUN L., HE Y., WANG X. Manure application affects microbial metabolic quotient through DOM recalcitrance and microbial strategy shifts in a mollisol. Soil and Tillage Research. 252, 106616, 2025.
 
37.
ZENG X., FENG J., CHEN J., DELGADO-BAQUERIZO M., ZHANG Q., ZHOU X., YUAN Y., FENG S., ZHANG K., LIU Y., HUANG Q. Microbial assemblies associated with temperature sensitivity of soil respiration along an altitudinal gradient. Science of The Total Environment. 820, 153257, 2022.
 
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