The effect of influence of static magnetic field which is widespread in environment is the aim of many studies. Static magnetic field is present in our everyday life and can cause functional disorders in cells, tissues or biological systems. The study’s results do not give the answer about negative or positive effect of the static magnetic field on human body. On the one hand, the magnetic field has been successfully used in medicine for many years, in the diagnosis and treatment of diseases of the musculoskeletal system, nervous system, eyesight, upper respiratory tract, circulatory system, gastrointestinal tract, skin and soft tissues. To induce oxidative stress, fibroblasts had been treated with hydrogen peroxide at a concentration of 1 mM and 0.5 mM for 4 hours, then the medium was changed to medium without of the hydrogen peroxide and cells were placed in permanent magnets with 0.7 T induction. The cultures were maintained in the test chambers or placebo at 37ºC in a 5% CO₂ incubator (Heraeus) for 24 hours. In cells lysates SOD, GPx, CAT activities were measured and also total antioxidant status. There was statistically significant decrease in the SOD and GPx activity in cultures with hydrogen peroxide addition. In the cultures to which hydrogen peroxide had been added, there was a statistically significant increase in the CAT activity. The simultaneous exposure of the fibroblasts to oxidative stress and the SMF caused a statistically significant increase in the SOD and GPx activity and decrease CAT activities when compare to the cultures exposed to hydrogen peroxide. The results showed that hydrogen peroxide causes oxidative stress in fibroblasts. On the other hand, the static magnetic field protects the cells and removes the harmful effects of hydrogen peroxide on the fibroblasts.