About the Journal
In Memory of Professor Jerzy Radecki
Editorial Office
Editorial Board
Scope
Impact Factor
Indexed in...
Journal Impact Factor contributing items
Contact
Subscription
Current issue
Online first
Instructions for Authors
Frequently Asked Questions
Editorial policies
PJOES Publication Policy Overview
PJOES Peer Review Policy
PJOES Research Ethics and Malpractice Policy
PJOES Plagiarism Policy
PJOES Conflict of Interest Policy
PJOES Open Access Policy
PJOES Instructions for Reviewers
Generative AI and AI-Assisted Technologies Policy
Archive
ORIGINAL RESEARCH
Exploring the Pollen Fertility Estimation
of Selected Taxa of Boraginaceae from Pakistan
1
Department of Plant Sciences, Quaid- i- Azam University Islamabad, 45320, Pakistan
2
Institute of Botany Academy Sciences Republic of Uzbekistan, Tashkent, 100125, Uzbekistan
3
Joint International Research Laboratory of Metabolic & Developmental Sciences, School
of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
4
Department of Botany and Microbiology, College of Science, King Saud University,
P.O. 2455, Riyadh 11451, Saudi Arabia
5
Department of Agronomy, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur
63100, Pakistan
6
Department of Life Sciences, Western Caspian University, Baku, Azerbaijan
7
Navoi State Pedagogical Institute, Navai, 210100, Uzbekistan
Submission date: 2024-08-03
Final revision date: 2024-10-10
Acceptance date: 2025-01-27
Online publication date: 2025-03-27
Corresponding author
KEYWORDS
TOPICS
ABSTRACT
This research aimed to evaluate the pollen fertility and micromorphology of Boraginaceae
species across Pakistan, with an emphasis on how population size influences reproduction. A total
of 27 species of the Boraginaceae family were collected from Pakistan, and light microscopy was
used to examine pollen parameters, including size, shape, exine thickness, and fertility percentage.
The findings revealed a range of pollen shapes, including prolate, prolate-spheroidal, sub-prolate, oblate,
sub-oblate, and oblate-spheroidal, with oblate and sub-oblate being the most frequent. Exine thickness
varied significantly among species, with Alkanna tinctoria having the thickest exine (2.80 μm), while
Onosma hispida exhibited the thinnest exine (0.75 μm). These variations in pollen shapes and exine
thickness play a crucial role in species identification and may influence reproductive success, as thicker
exines offer structural protection against UV radiation, desiccation, and microbial attack, enhancing
pollen survival during pollination. Fertility rates also varied, with Trichodesma indicum exhibiting
the highest pollen fertility rate (94.94%), whereas Arnebia hispidissima had the lowest (60.50%) with
an average fertility of 78.83% across the species. Larger populations exhibited higher pollen viability,
while small, isolated populations had reduced fertility, demonstrating the significant effect of population
size on reproduction. This study uncovers previously undocumented pollen fertility and morphology differences among Boraginaceae species in Pakistan, adding valuable information to current knowledge
and emphasizing elements that influence reproductive success.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
REFERENCES (44)
1.
SADIA B., AHMAD M., ULLAH F., ZAFAR M., SULTANA S., ALREFAEI A.F., ZAMAN W. Desert Blooms: Unraveling Palyno-Anatomical Diversity In Arid Boraginaceous Taxa. Pakistan Journal of Botany, 56 (5), 1929, 2024. https://doi.org/10.30848/PJB20...).
2.
JONES R.L., PÉREZ Á.J., REYNOLDS A., WEBSTER G.L. Botanical inventory and conservation assessment of Siempre Verde Reserve, Imbabura Province, Ecuador. Journal of the Botanical Research Institute of Texas, 17 (2), 457, 2023. https://doi.org/10.17348/jbrit....
3.
AHMAD S., AHMAD M., FAWZY RAMADAN M., SULTANA S., PAPINI A., ULLA F., ZAFAR M. Palynological study of fossil plants from Miocene Murree Formation of Pakistan: Clues to investigate palaeoclimate and palaeoenvironment. Agronomy, 13 (1), 269, 2023. https://doi.org/10.3390/agrono....
4.
NOOR W., ZAFAR M., AHMAD M., SADIA B., GILLANI S.W., MANZOOR M., AMEEN M. Taxonomic significance of palyno-morphic markers for the delimitation of some Brassicaceous taxa in Balochistan Province (Pakistan). Genetic Resources and Crop Evolution, 1, 2024. https://doi.org/10.1007/s10722....
5.
HAQ A., BADSHAH L. Floristic description and ecological characteristics of the plants of Pashat Valley, Pak-Afghan border, district Bajaur, Pakistan. Acta Ecologica Sinica, 41 (6), 524, 2021. https://doi.org/10.1016/j.chna....
6.
ARSHAD F., HAQ S.M., WAHEE M., JAMEEL M.A., BUSSMANN R.W. Environmental variables drive medicinal plant composition and distribution in various forest types of subtropical region in Pakistan. Ecological Frontiers, 44 (2), 234, 2024. https://doi.org/10.1016/j.chna....
7.
ASADI-SAMANI M., BAHMANI M., RAFIEIAN-KOPAEI M. The chemical composition, botanical characteristic and biological activities of Borago officinalis: a review. Asian Pacific Journal of Tropical Medicine, 7, S22, 2014. https://doi.org/10.1016/S1995-... PMid:25312125.
8.
NOCENTINI D., PACINI E., GUARNIERI M., NEPI M. Flower morphology, nectar traits and pollinators of Cerinthe major (Boraginaceae-Lithospermeae). Flora-Morphology, Distribution, Functional Ecology of Plants, 207 (3), 186, 2012. https://doi.org/10.1016/j.flor....
9.
DE HARO A., DOMÍNGUEZ V., DEL RÍO M. Variability in the content of gamma-linolenic acid and other fatty acids of the seed oil of germplasm of wild and cultivated borage (Borago officinalis L.). Journal of Herbs, Spices & Medicinal Plants, 9 (4), 297, 2002. https://doi.org/10.1300/J044v0....
10.
OZA M.J., KULKARNI Y.A. Traditional uses, phytochemistry and pharmacology of the medicinal species of the genus Cordia (Boraginaceae). Journal of Pharmacy and Pharmacology, 69 (7), 755, 2017. https://doi.org/10.1111/jphp.1... PMid:28266011.
11.
DAFNI A., FIRMAGE D. Pollen viability and longevity: practical, ecological and evolutionary implications. Pollen and Pollination, 113, 2000. https://doi.org/10.1007/978-3-....
12.
PIPINO L., VAN LABEKE M.C., MANSUINO A., SCARIOT V., GIOVANNINI A., LEUS L. Pollen morphology as fertility predictor in hybrid tea roses. Euphytica, 178, 203, 2011. https://doi.org/10.1007/s10681....
13.
VAN DER MERWE M.M., BRAGG J.G., DIMON R., FAHEY P.S., HOGBIN P.M., LU-IRVING P., YAP J.Y.S. Maintaining separate maternal lines increases the value and applications of seed collections. Australian Journal of Botany, 2024. https://doi.org/10.1071/BT2213....
14.
LUNAU K., DE CAMARGO M.G.G., BRITO V.L.G. Pollen, anther, stamen, and androecium mimicry. Plant Biology, 26 (3), 349, 2023. https://doi.org/10.1111/plb.13... PMid:38407440.
16.
WESTERMANN J., SRIKANT T., GONZALO A., TAN H.S., BOMBLIES K. Defective pollen tube tip growth induces neo-polyploid infertility. Science, 383 (6686), 2024. https://doi.org/10.1126/scienc... PMid:38422152.
17.
LORA J., GARCIA-LOR A., ALEZA P. Pollen development and viability in diploid and doubled diploid citrus species. Frontiers in Plant Science, 13, 862813, 2024. https://doi.org/10.3389/fpls.2... PMid:35557738 PMCid:PMC9090487.
18.
WANG B., LIANG N., SHEN X., XIE Z., ZHANG L., TIAN B., WEI X. Cytological and transcriptomic analyses provide insights into the pollen fertility of synthetic allodiploid Brassica juncea hybrids. Plant Cell Reports, 43 (1), 23, 2024. https://doi.org/10.1007/s00299... PMid:38150101.
19.
IMPE D. Wheat pollen viability and physiological, biochemical and biophysical factors impacting pollen storability. 2022.
20.
ALTHIAB-ALMASAUD R., TEYSSIER E., CHERVIN C., JOHNSON M.A., MOLLET J.C. Pollen viability, longevity, and function in angiosperms: key drivers and prospects for improvement. Plant Reproduction, 1, 2023. https://doi.org/10.1007/s00497... PMid:37926761.
21.
ZHAO W., HOU Q., QI Y., WU S., WAN X. Structural and molecular basis of pollen germination. Plant Physiology and Biochemistry, 108042, 2023. https://doi.org/10.1016/j.plap... PMid:37738868.
22.
DENG W., LIN M.Z., TIAN H.Q. Callose wall and sporoderm characteristics during the anther development stage of Dendrobium officinale. Flora, 303, 152278, 2023. https://doi.org/10.1016/j.flor....
23.
AZEEM A., MAI W., ALI R. Modeling Plant Height and Biomass Production of Cluster Bean and Sesbania across Diverse Irrigation Qualities in Pakistan's Thar Desert. Water, 16 (1), 9-21, 2024. https://doi.org/10.3390/w16010....
24.
DILAWAR A., CHEN B., UL-HAQ Z., ALI S., SAJJAD M.M., JUNJUN F., LODHI E. Evaluating the potential footprints of land use and land cover and climate dynamics on atmospheric pollution in Pakistan. Frontiers in Environmental Science, 11, 1272155, 2024. https://doi.org/10.3389/fenvs.....
25.
SALEEM F., ARSHAD A., MIRCHI A., KHALIQ T., ZENG X., RAHMAN M.M., MAHMOOD K. Observed changes in crop yield associated with droughts propagation via natural and human-disturbed agro-ecological zones of Pakistan. Remote Sensing, 14 (9), 2152, 2022. https://doi.org/10.3390/rs1409....
26.
SINGH D.K., PUSALKAR P.K. Floristic diversity of the Indian Himalaya. Biodiversity of the Himalaya: Jammu and Kashmir State, 93, 2022. https://doi.org/10.1007/978-98....
27.
AHMED K., SHAHID S., WANG X., NAWAZ N., KHAN N. Spatiotemporal changes in aridity of Pakistan during 1901-2016. Hydrology and Earth System Sciences, 23 (7), 3081-3096, 2019. https://doi.org/10.5194/hess-2....
28.
KILIÇ T. Identifying successful combinations by fertility index in old garden roses and hybrid tea roses crosses. PeerJ, 11, e15526, 2023. https://doi.org/10.7717/peerj.... PMid:37361039 PMCid:PMC10286800.
29.
DA SILVA T.A., NASCIMENTO G.Q.S.D.S., DE ALMEIDA P.S., CAVALCANTE B.P., SOUZA F.V.D., AONA L.Y.S., DE SOUZA E.H. Pollen morphology, viability, and stigma receptivity of Hohenbergia (Bromeliaceae) species occurring in the Atlantic Forest biome with potential for landscaping and cut flowers. Genetic Resources and Crop Evolution, 1, 2024. https://doi.org/10.1007/s10722....
30.
LIU Q., YANG J., WANG X., ZHAO Y. Studies on pollen morphology, pollen vitality and preservation methods of Gleditsia sinensis Lam.(Fabaceae). Forests, 14 (2), 243, 2023. https://doi.org/10.3390/f14020....
31.
AHMAD K., SHAHEEN N., AHMAD M., KHAN M.A. Pollen fertility estimation of some sub-tropical flora of Pakistan. African Journal of Biotechnology, 9 (49), 8313, 2010.
32.
MAZARI P., LIU Q., KHAN M.A., SADIA S., AHMAD L. Pollen morphology and pollen fertility estimation of three medicinal plant species of Hypericum L. from Kaghan Valley, Northern Pakistan. American Journal of Plant Sciences, 8 (12), 3073, 2017. https://doi.org/10.4236/ajps.2....
33.
USMA A., AHMAD M., RAMADAN M.F., KHAN A.M., ZAFAR M., HAMZA M., YASEEN G. Micro‐morphological diversity of pollen among Asteraceous taxa from Potohar Plateau‐Pakistan. Microscopy Research and Technique, 85 (7), 2467, 2022. https://doi.org/10.1002/jemt.2... PMid:35294076.
34.
KUMAR A., SHARMA S.S., KUMAR B. Palynological studies on some plants of Boraginaceae. Journal of Biotechnology and Biochemistry, 6, 4, 2020.
35.
BOCHYNEK T., BURD M. Pollination efficiency and the pollen-ovule ratio. New Phytologist, 2024. https://doi.org/10.1111/nph.19... PMid:38937955.
36.
OSMAN K., DESJARDINS S.D., SIMMONDS J., BURRIDGE A.J., KANYUKA K., HENDERSON I.R., SANCHEZ‐MORAN E. FIGL1 prevents aberrant chromosome associations and fragmentation and limits crossovers in polyploid wheat meiosis. New Phytologist, 2024. https://doi.org/10.1111/nph.19... PMid:38584326.
37.
KÄMPER W., NICHOLS J., TRAN T.D., BURWELL C.J., BYRNES S., TRUEMAN S.J. Flower visitors, levels of cross-fertilisation, and pollen-parent effects on fruit quality in mango orchards. Agronomy, 13 (10), 2568, 2023. https://doi.org/10.3390/agrono....
38.
NAZISH M., BIBI F., ALWAHIBI M.S., ELSHIKH M.S., ZAMAN W. Exploration of salinity effect on palyno-morphological characteristics of plant species collected from salt range. Bangladesh Journal of Plant Taxonomy, 31 (1), 33, 2024. https://doi.org/10.3329/bjpt.v....
39.
AHMAD S., ZAFAR M., AHMAD M., LUBNA, YASEEN G., SULTANA S. Microscopic investigation of palyno‐morphological features of melliferous flora of Lakki Marwat district, Khyber Pakhtunkhwa, Pakistan. Microscopy Research and Technique, 82 (6), 720, 2019. https://doi.org/10.1002/jemt.2... PMid:30676673.
40.
AHMAD S., ZAFAR M., AHMAD M., OZDEMIR F.A., YASEEN G., SULTANA S., KUTLU, M.A. Palynological studies of winter weeds melliferous flora of district Bannu, Khyber Pakhtunkhwa, Pakistan. Annali di Botanica, 77, 2020.
41.
AHMAD S., ZAFAR M., AHMAD M., SULTANA S., MAJEED S., YASEEN G. Morpho-Palynological investigations of melliferous plants using microscopic techniques from Khyber Pakhtunkhwa, Pakistan. Acta Botanica Hungarica, 64 (1), 2022. https://doi.org/10.1556/034.64....
42.
AHMAD S., ZAFAR M., AHMAD M., ZAFAR S., ARFAN M., KHAN A.M., SULTANA S., ABBASI M.A., MAJEED S. Melissopalynological studies of autumn honey samples from Khyber Pakhtunkhwa, Pakistan. Journal of Animal and Plant Sciences, 33, 1184, 2023. https://doi.org/10.36899/JAPS.....
43.
ULLAH A., AHMAD M., IQBAL Z., AHMAD S., PIERONI A., SHAH H., ISLAMOV S., ULLAH R., USMANKULOVNA N.M., MAKHKAMOV T., ULLAH T. Ultra-sculpturing of nutlet morphology and its taxonomic significance for identifications of Boraginaceae species. Genetic Resources and Crop Evolution, pp. 1, 2024. https://doi.org/10.1007/s10722....
44.
ULLAH S., ZAFAR M., PTASZYŃSKA A.A., AHMAD M., AHMAD S., ULLAH A., SHAH H., AL-ANAZI K.M., FARAH M.A., MAKHKAMOV T., SOTIBOLDIYEVA D. Taxonomic implications of pollen morphological features for taxa identifications: plant-honeybee's interactions. Genetic Resources and Crop Evolution, pp.1, 2024. https://doi.org/10.1007/s10722....
Share
RELATED ARTICLE
| eISSN: | 2083-5906 |
| ISSN: | 1230-1485 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
