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ORIGINAL RESEARCH
An Investigation and Trapping Wild Colonies
of the Black Soldier Fly, Morphological
Identification of Larval Stages Under
a Controlled Environment in Pakistan
1
Department of Zoology, Abdul Wali Khan University Mardan
2
College of Veterinary Science and Animal Husbandry, Abdul Wali Khan University Mardan
3
Department of Botany, Abdul Wali Khan University Mardan
4
Institute of Chemical Sciences, University of Peshawar, Pakistan
5
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
6
Department of Horticulture and Life Science, Yeungnam University, Republic of Korea
Submission date: 2023-11-08
Final revision date: 2024-04-02
Acceptance date: 2024-04-18
Online publication date: 2024-09-02
Publication date: 2025-01-28
Corresponding author
Pol. J. Environ. Stud. 2025;34(3):2461-2472
KEYWORDS
TOPICS
ABSTRACT
The black soldier fly larvae Hermetia illucens L. (Diptera: Stratiomyidae) are an ideal insect to
produce compost and animal feed. The goal of this study was to use Kitchen waste 50 grams, Cow dung
50 grams, and Rotten fruits 50 grams attractants to gather wild H. illucens and their egg clutches from
their natural microhabitat in dumping sites. The fly was trapped in a 1×1×1 feet trap net. The results
indicate that humidity 42.5±50.5%, F = 1.754, P = .233 and temperature 27.975±37.05ºC, F = 1.126,
P = .353) during the capture of H. illucens and egg clutch in a natural environment. The life cycle has
included seven instars, a duration of 39 days, and the longest time period of the instar 6th recorded.
We have optimized the temperature range to 24-30ºC and humidity to 60-95% in indoor rearing
of the H. illucens. We recorded the varying ingredient contents level, highest Moisture at 38.5%, Crude
protein at 55.13%, Crude fiber at 28.18%, Crude fat at 28.18%, and Total Ash at 15.5%. The H. illucens
indoor farming is recommended for quality of protein production under a controlled environment which
will boost the Pakistani economy and reduce threats to aquatic fauna.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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| eISSN: | 2083-5906 |
| ISSN: | 1230-1485 |
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